Supplementary Materials

Supplementary Material for:

Photoactivation of Endogenous Latent Transforming Growth Factor–β1 Directs Dental Stem Cell Differentiation for Regeneration

Praveen R. Arany, Andrew Cho, Tristan D. Hunt, Gursimran Sidhu, Kyungsup Shin, Eason Hahm, George X. Huang, James Weaver, Aaron Chih-Hao Chen, Bonnie L. Padwa, Michael R. Hamblin, Mary Helen Barcellos-Hoff, Ashok B. Kulkarni, David J. Mooney*

*Corresponding author. E-mail:

Published 28 May 2014, Sci. Transl. Med. 6, 238ra69 (2014)
DOI: 10.1126/scitranslmed.3008234

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of tertiary dentin induction by histology and μCT.
  • Fig. S2. Raman mapping for compositional assessment of tooth mineralized tissue.
  • Fig. S3. LPL-generated ROS in a cell-free system.
  • Fig. S4. LPL activation of LTGF-β1 is mediated by ROS.
  • Fig. S5. Characterization of hDSCs.
  • Fig. S6. Culture system for evaluation of the LPL–ROS–TGF-β1 axis.
  • Fig. S7. Release kinetics of TGF-βRI inhibitor from microspheres.
  • Fig. S8. Characterization of DSPPCreTGFRIIfl/fl conditional knockout mice.
  • Fig. S9. LPL treatment of TGFRIIfl/fl wild-type mice.
  • Table S1. Reagents used to generate and detect ROS.
  • Table S2. Primers and conditions for semiquantitative RT-PCR.
  • Table S3. List of antibodies for immunoassays.

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