Supplementary Materials

Supplementary Material for:

Merlin Deficiency Predicts FAK Inhibitor Sensitivity: A Synthetic Lethal Relationship

Irina M. Shapiro, Vihren N. Kolev, Christian M. Vidal, Yuwaraj Kadariya, Jennifer E. Ring, Quentin Wright, David T. Weaver, Craig Menges, Mahesh Padval, Andrea I. McClatchey, Qunli Xu, Joseph R. Testa, Jonathan A. Pachter*

*Corresponding author. E-mail:

Published 21 May 2014, Sci. Transl. Med. 6, 237ra68 (2014)
DOI: 10.1126/scitranslmed.3008639

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Chemical structure of VS-4718.
  • Fig. S2. Western blot analysis of lysates from cancer cell lines in Fig. 1A.
  • Fig. S3. pFAK-Y397 levels in MDA-MB-231 and MDA-MB-468 xenograft tumor samples.
  • Fig. S4. Average EC50 values for Fig. 2B.
  • Fig. S5. Relative growth of MDA-MB-231/GFP and MDA-MB-231/NF2-GFP cells in response to VS-4718.
  • Fig. S6. Quantitative polymerase chain reaction analysis of FAK cDNA in MSTO-211H and Mero-41 cells.
  • Fig. S7. TGI and pFAK-Y397 expression analysis in MSTO-211H xenograft model.
  • Fig. S8. Merlin and pFAK-Y397 expression in human mesothelioma PDX models.
  • Fig. S9. pAKT and pFAK activation in Mero-48a and MSTO-211H cell lines.
  • Fig. S10. pFAK-Y397 immunofluorescence analysis in Mero-41 and Mero-48a cells expressing NF2-GFP.
  • Fig. S11. N-cadherin–blocking antibody treatment in H28 and MSTO-211H cells.
  • Fig. S12. Quantification of pFAK-Y397 in H28, Mero-25, Mero-83, and Mero-41 cells.
  • Fig. S13. Tumorsphere self-renewal and in vivo differentiation analysis of Aldefluor+ MM87 cells.
  • Fig. S14. Effect of VS-4718 treatment on Aldefluor+ CSCs in Mero-83 and Mero-48a MPM cells.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Original data from in vivo studies (provided as an Excel file).