Supplementary Materials

Supplementary Material for:

PHD Inhibition Mitigates and Protects Against Radiation-Induced Gastrointestinal Toxicity via HIF2

Cullen M. Taniguchi, Yu Rebecca Miao, Anh N. Diep, Colleen Wu, Erinn B. Rankin,
Todd F. Atwood, Lei Xing, Amato J. Giaccia*

*Corresponding author. E-mail: giaccia{at}stanford.edu

Published 14 May 2014, Sci. Transl. Med. 6, 236ra64 (2014)
DOI: 10.1126/scitranslmed.3008523

This PDF file includes:

  • Materials and Methods
  • Fig. S1. TAI requires a custom jig.
  • Fig. S2. Single and double PHD knockout combinations are not radioprotective.
  • Fig. S3. Radiation and DMOG both induce HIF in normal tissues.
  • Fig. S4. DMOG radioprotects over a range of doses.
  • Fig. S5. Jejunal cross sections are used for crypt counting.
  • Fig. S6. DMOG increased regenerating crypts and decreased apoptosis in the colon after TAI.
  • Fig. S7. γH2AX staining in the intestine is not different between saline and DMOG mice after TAI.
  • Fig. S8. DMOG mildly increases hematocrit after TAI.
  • Fig. S9. Tumors are not radioprotected by PHD inhibition.
  • Fig. S10. Mouse diarrhea is recognized as loose stools and lack of formed stools.
  • Fig. S11. Metabolic cage measurements quantified intake and waste products from each mouse.
  • Fig. S12. VEGF and angiogenesis are involved in radioprotection.
  • Fig. S13. VEGF inhibition by Flt1 increases hematocrit.
  • Fig. S14. LSL–Villin-Cre animals express HIF in the colon.
  • Fig. S15. Necropsies of long-term survivors reveal no major abnormalities.
  • Fig. S16. Radiation induced fatty change in tibia bone marrow of long-term survivors.
  • Fig. S17. Humerus sections from aged survivors are indistinguishable from agematched controls.
  • Fig. S18. DMOG mitigates radiation toxicity when administered 4 hours after radiation exposure.
  • Table S2. Complete blood count measurements at 5 days after radiation showing no differences between saline and DMOG treatments.
  • Table S3. TAI-induced hypernatremia and hyperglycemia in saline controls.
  • Table S4. Anemia in long-term survivors of TAI compared to unirradiated controls.
  • References (52, 53)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Original data (provided as an Excel file).