Supplementary Materials

Supplementary Material for:

CpG ODN Nanorings Induce IFNα from Plasmacytoid Dendritic Cells and Demonstrate Potent Vaccine Adjuvant Activity

Bilgi Gungor, Fuat Cem Yagci, Gizem Tincer, Banu Bayyurt, Esin Alpdundar, Soner Yildiz, Mine Ozcan, Ihsan Gursel, Mayda Gursel*

*Corresponding author. E-mail: mgursel@metu.edu.tr

Published 7 May 2014, Sci. Transl. Med. 6, 235ra61 (2014)
DOI: 10.1126/scitranslmed.3007909

This PDF file includes:

  • Method
    Fig. S1. Secondary structure predictions of the 20-mer CpG ODN (left) and the 12-mer CpG ODN (right) were generated using the M-fold program.
  • Fig. S2. Physical properties of K-ODN/cationic peptide complexes prepared using various ODN/peptide ratios.
  • Fig. S3. IFNα-inducing activity does not correlate with particle size.
  • Fig. S4. IFNα-stimulating activity of various K-ODN/cationic peptide complexes in hPBMCs.
  • Fig. S5. K-ODN/cationic peptide complexes are not cytotoxic and do not trigger inflammasome activation.
  • Fig. S6. K-ODN/cationic peptide complexes trigger TNFα production from pDCs.
  • Table S1. Average particle size, PI, and ζ potential measurements of complexes prepared with the 12- and 20-mer K-ODN.
  • Table S2. Raw data and statistical significance testing for Fig. 2A.
  • Table S3. Raw data and statistical significance testing for Fig. 3A.
  • Table S4. Raw data and statistical significance testing for Fig. 3C.
  • Table S5. Results of Tukey’s HSD post hoc test using one-way ANOVA for Fig. 5.

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