Supplementary Materials

Supplementary Material for:

Modular Multiantigen T Cell Epitope–Enriched DNA Vaccine Against Human Leishmaniasis

Shantanabha Das, Anja Freier, Thouraya Boussoffara, Sushmita Das, Detlef Oswald, Florian O. Losch, Melanie Selka, Nina Sacerdoti-Sierra, Gabriele Schönian, Karl-Heinz Wiesmüller, Karin Seifert, Matthias Schroff, Christiane Juhls, Charles L. Jaffe, Syamal Roy, Pradeep Das, Hechmi Louzir, Simon L. Croft, Farrokh Modabber, Peter Walden*

*Corresponding author. E-mail:

Published 30 April 2014, Sci. Transl. Med. 6, 234ra56 (2014)
DOI: 10.1126/scitranslmed.3008222

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Procedure for the selection of vaccine antigens in LEISHDNAVAX.
  • Fig. S2. Sequence comparisons.
  • Fig. S3. Peptide pools for epitope scans.
  • Fig. S4. Specificity of T cells induced in leishmaniasis.
  • Fig. S5. Sequence comparison of vaccines.
  • Fig. S6. Western blot analysis for protein expression by MIDGE-TH1–transfected cells.
  • Fig. S7. Frequencies of IL-2–producing CD4 T cells.
  • Fig. S8. Frequencies of IL-2–producing CD8 T cells.
  • Fig. S9. Proliferation of T cells from mice vaccinated with LEISHDNAVAX.
  • Fig. S10. Parasite burden in liver and spleen of mice vaccinated or not with LEISHDNAVAX and challenged with L. donovani after 90 days of infection.
  • Table S1. Leishmania isolates analyzed for sequence variations in the candidate vaccine antigens.
  • Table S2. PCR protocol for amplifying the vaccine antigen genes from genomic DNA of Leishmania isolates.

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