Supplementary Materials

Supplementary Material for:

Suppression of p21Rac Signaling and Increased Innate Immunity Mediate Remission in Crohn’s Disease

Kaushal Parikh, Lu Zhou, Rajesh Somasundaram, Gwenny M. Fuhler, J. Jasper Deuring, Tjasso Blokzijl, Anouk Regeling, Ernst J. Kuipers, Rinse K. Weersma, Veerle J. Nuij, Maria Alves, Lauran Vogelaar, Lydia Visser, Colin de Haar, Kausilia K. Krishnadath, C. Janneke van der Woude, Gerard Dijkstra, Klaas Nico Faber, Maikel P. Peppelenbosch*

*Corresponding author. E-mail:

Published 23 April 2014, Sci. Transl. Med. 6, 233ra53 (2014)
DOI: 10.1126/scitranslmed.3006763

This PDF file includes:

  • Methods
  • Fig. S1. Confirmation of signaling differences in CD identified by PepChip analysis.
  • Fig. S2. Magnification of PAKSer20 and Rac-GTP IHC.
  • Fig. S3. Age of patients and location of biopsy in colon do not affect Rac activity.
  • Fig. S4. Macrophages, plasma cells, and neutrophils show changes in PAK phosphorylation.
  • Fig. S5. Increased PAK2Ser20 staining in peripheral blood phagocytes from CD patients.
  • Fig. S6. GEF assay and effect of NSC23766 on p21Rac1 activity.
  • Fig. S7. 6-TG reduces GTP-loaded Rac1 in monocytes from healthy individuals.
  • Fig. S8. IL-8 mRNA expression in colonic biopsies.
  • Legends for tables S1 to S3

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Patients used for the biochemical experiments.
  • Table S2 (Microsoft Excel format). Kinome results in CD UC.
  • Table S3 (Microsoft Excel format). Genotyping information for patients.

[Tables S1 to S3]