Supplementary Materials

Supplementary Material for:

Immunological Visibility: Posttranscriptional Regulation of Human NKG2D Ligands by the EGF Receptor Pathway

Pierre Vantourout, Carrie Willcox, Andrea Turner, Chad M. Swanson, Yasmin Haque, Olga Sobolev, Anita Grigoriadis, Andrew Tutt, Adrian Hayday*

*Corresponding author. E-mail: adrian.hayday@kcl.ac.uk; adrian.hayday@cancer.org.uk

Published 9 April 2014, Sci. Transl. Med. 6, 231ra49 (2014)
DOI: 10.1126/scitranslmed.3007579

This PDF file includes:

  • Fig. S1. Induction of MICA by UVB and EGF is not due to heat shock, DNA damage, or cell proliferation.
  • Fig. S2. NKG2D ligands are up-regulated at the cell surface after EGF treatment.
  • Fig. S3. Cell surface increase in NKG2D ligand expression by EGF is detected by cytotoxic lymphocytes.
  • Fig. S4. NKG2D ligand induction by various stress components of the exposome is EGFR-dependent.
  • Fig. S5. NKG2D ligand expression is regulated posttranscriptionally.
  • Fig. S6. NKG2D ligand mRNAs contain ARE sequences in their 3′UTRs.
  • Fig. S7. The ARE sequence and the MEK pathway regulate NKG2D ligand expression.
  • Fig. S8. AUF1 regulates NKG2D ligand expression.
  • Fig. S9. The EGFR/MEK pathway regulates AUF1 localization.
  • Fig. S10. NKG2D ligand expression correlates with EGFR expression levels and is abrogated by erlotinib.
  • Fig. S11. EGF-induced up-regulation of cell surface NKG2D ligand expression by confluent differentiated Caco-2 cells is abrogated by EGFR and MEK inhibitors.
  • Fig. S12. Cetuximab inhibits NKG2D ligand expression.
  • Table S1. Primers used in this study.

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