Supplementary Materials

Supplementary Material for:

Cell Distance Mapping Identifies Functional T Follicular Helper Cells in Inflamed Human Renal Tissue

Vladimir M. Liarski, Natalya Kaverina, Anthony Chang, Daniel Brandt, Denisse Yanez, Lauren Talasnik, Gianluca Carlesso, Ronald Herbst, Tammy O. Utset, Christine Labno, Yahui Peng, Yulei Jiang, Maryellen L. Giger, Marcus R. Clark*

*Corresponding author. E-mail:

Published 2 April 2014, Sci. Transl. Med. 6, 230ra46 (2014)
DOI: 10.1126/scitranslmed.3008146

This PDF file includes:

  • Fig. S1. TFH ICOS+ cells in human LuN are limited to the renal tubulointerstitium.
  • Fig. S2. Preliminary development and validation of automated CDM method.
  • Fig. S3. Relationship between cell density and observed TFH–B cell conjugate rates.
  • Fig. S4. Immunofluorescence analysis of MR biopsies reveals different patterns of TFH–B cell distribution.
  • Fig. S5. Comparison of CDM results to a control random distribution model.
  • Fig. S6. TFH ICOS+ cells in human LuN produce IL-21 but not IL-17.
  • Fig. S7. Original antibody staining used for three-dimensional surface creation of TFH–B cell cognate pairs in LuN.
  • Table S1. LuN patient cohort clinical and histological characteristics.
  • Table S2. Digital image database used for CDM analysis.
  • Table S3. Direct statistical comparison of CDM distributions between clinical biopsy groups.
  • Table S4. Curve fit analysis for assessing kinetics of CDM distributions between cases.

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