Supplementary Materials

Supplementary Material for:

IL-18 Attenuates Experimental Choroidal Neovascularization as a Potential Therapy for Wet Age-Related Macular Degeneration

Sarah L. Doyle,* Ema Ozaki, Kiva Brennan, Marian M. Humphries, Kelly Mulfaul, James Keaney, Paul F. Kenna, Arvydas Maminishkis, Anna-Sophia Kiang, Sean P. Saunders, Emily Hams, Ed C. Lavelle, Clair Gardiner, Padraic G. Fallon, Peter Adamson, Peter Humphries, Matthew Campbell*

*Corresponding author. E-mail: matthew.campbell@tcd.ie (M.C.); sarah.doyle@tcd.ie (S.L.D.)

Published 2 April 2014, Sci. Transl. Med. 6, 230ra44 (2014)
DOI: 10.1126/scitranslmed.3007616

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Analysis of human NK cells.
  • Fig. S2. Effect of IL-18 on RPE cell integrity.
  • Fig. S3. Viability of RPE cells after IL-18 treatment.
  • Fig. S4. Differentially regulated NFκB-associated genes 6 hours after IL-18 treatment.
  • Fig. S5. Differentially regulated NFκB-associated genes 24 hours after IL-18 treatment.
  • Fig. S6. Decrease in angiogenesis-associated proteins and cytokines after IL-18 treatment.
  • Fig. S7. Primary RPE cell characterization.
  • Fig. S8. No apoptosis or necrosis of RPE cells after treatment with IL-18.
  • Fig. S9. ZO-1 expression in RPE cells.
  • Fig. S10. Western blot and densitometric quantification of ZO-1 in primary RPE cells.
  • Fig. S11. Human RPE cell response to pro–IL-18 and pro–IL-1b.
  • Fig. S12. Cell swelling after expression of pro–IL-18.
  • Fig. S13. Pro–IL-18 expression in RPE for 3 months.
  • Fig. S14. Necrosis gene array after transfection of RPE cells with pro–IL-18.
  • Fig. S15. Necrosis gene array after transfection of RPE cells with pro–IL-1b.
  • Fig. S16. Mouse ASC-cerulean oligomerization.
  • Fig. S17. Autophagy prevents RPE cell swelling induced by pro–IL-18 expression.
  • Fig. S18. Bone marrow chimera reconstitution.
  • Fig. S19. IL-18 injected 1 day before CNV has no effect on lesion volume.

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