Supplementary Materials

Supplementary Material for:

Cytotoxicity of Paclitaxel in Breast Cancer Is due to Chromosome Missegregation on Multipolar Spindles

Lauren M. Zasadil, Kristen A. Andersen, Dabin Yeum, Gabrielle B. Rocque, Lee G. Wilke, Amye J. Tevaarwerk, Ronald T. Raines, Mark E. Burkard, Beth A. Weaver*

*Corresponding author. E-mail: baweaver@wisc.edu

Published 26 March 2014, Sci. Transl. Med. 6, 229ra43 (2014)
DOI: 10.1126/scitranslmed.3007965

This PDF file includes:

  • Fig. S1. Mitotic index is >15-fold elevated between 16 and 32 hours after paclitaxel administration in breast cancer cells in culture.
  • Fig. S2. Clinically relevant concentrations of paclitaxel cause abnormal mitoses in MDA-MB-231 cells.
  • Fig. S3. Clinically relevant doses of paclitaxel do not disrupt Golgi structure.
  • Fig. S4. Clinically relevant doses of paclitaxel do not cause substantial cell death in interphase.
  • Fig. S5. The proliferative index in patient tumors is unchanged by paclitaxel treatment.
  • Table S1. Paclitaxel measurements in patients by tumor volume.
  • Table S2. Statistical P values from Figs. 3 and 4.
  • Legends for videos S1 to S6

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Other Supplementary Material for this manuscript includes the following:

  • Video S1 (.mov format). Normal bipolar division in a control Cal51 breast cancer cell.
  • Video S2 (.mov format). Abnormal division in a Cal51 cell treated with 10 nM paclitaxel.
  • Video S3 (.mov format). Abnormal division in a Cal51 cell treated with 10 nM paclitaxel.
  • Video S4 (.mov format). Normal division in an MDA-MB-231 cell.
  • Video S5 (.mov format). Abnormal division in an MDA-MB-231 cell treated with 5 nM paclitaxel.
  • Video S6 (.mov format). Abnormal division in an MDA-MB-231 cell treated with 10 nM paclitaxel.

[Videos S1 to S6]