Supplementary Materials

Supplementary Material for:

Localized Oncolytic Virotherapy Overcomes Systemic Tumor Resistance to Immune Checkpoint Blockade Immunotherapy

Dmitriy Zamarin,* Rikke B. Holmgaard, Sumit K. Subudhi, Joon Seok Park, Mena Mansour, Peter Palese, Taha Merghoub, Jedd D. Wolchok, James P. Allison*

*Corresponding author. E-mail: zamarind@mskcc.org (D.Z.); jallison@mdanderson.org (J.P.A.)

Published 5 March 2014, Sci. Transl. Med.6, 226ra32 (2014)
DOI: 10.1126/scitranslmed.3008095

This PDF file includes:

  • Materials and Methods
  • Fig. S1. NDV infection is restricted to the injected tumor.
  • Fig. S2. NDV infection increases tumor leukocyte infiltration in the virus-injected tumors.
  • Fig. S3. NDV therapy increases distant tumor lymphocyte infiltration in bilateral footpad melanoma model.
  • Fig. S4. Intratumoral NDV provides protection from tumor rechallenge.
  • Fig. S5. Tumor-infiltrating CD8+ lymphocytes up-regulate CTLA-4 in response to NDV therapy.
  • Fig. S6. NDV and CTLA-4 synergize to reject local and distant tumors in the CT26 colon carcinoma model.
  • Fig. S7. Combination therapy with NDV and anti–CTLA-4 enhances tumor infiltration with innate and adaptive immune cells.
  • Fig. S8. Antibodies to CD8, CD4, and NK1.1 deplete the cells of interest in vivo.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data and statistical testing for Fig. 1, C to K.
  • Table S2 (Microsoft Excel format). Raw data and statistical testing for Fig. 1, L and M.
  • Table S3 (Microsoft Excel format). Raw data and statistical testing for Fig. 2, C and D.
  • Table S4 (Microsoft Excel format). Raw data and statistical testing for Fig. 2, E to I.
  • Table S5 (Microsoft Excel format). Raw data and statistical testing for Fig. 3, B to E.
  • Table S6 (Microsoft Excel format). Raw data and statistical testing for Fig. 3, F and G.
  • Table S7 (Microsoft Excel format). Raw data and statistical testing for Fig. 4.
  • Table S8 (Microsoft Excel format). Raw data and statistical testing for Fig. 5.
  • Table S9 (Microsoft Excel format). Raw data and statistical testing for Fig. 6.

[Tables S1 to S9]