Supplementary Materials

Supplementary Material for:

A Zebrafish Compound Screen Reveals Modulation of Neutrophil Reverse Migration as an Anti-Inflammatory Mechanism

Anne L. Robertson, Geoffrey R. Holmes, Aleksandra N. Bojarczuk, Joseph Burgon, Catherine A. Loynes, Myriam Chimen, Amy K. Sawtell, Bashar Hamza, Joseph Willson, Sarah R. Walmsley, Sean R. Anderson, Mark C. Coles, Stuart N. Farrow, Roberto Solari, Simon Jones, Lynne R. Prince, Daniel Irimia, G. Ed Rainger, Visakan Kadirkamanathan, Moira K. B. Whyte, Stephen A. Renshaw*

*Corresponding author. E-mail: s.a.renshaw@sheffield.ac.uk

Published 26 February 2014, Sci. Transl. Med. 6, 225ra29 (2014)
DOI: 10.1126/scitranslmed.3007672

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Positive hit compounds significantly reduce neutrophil numbers at 12 hpi to accelerate inflammation resolution.
  • Fig. S2. A subset of the positive hit compounds inhibits neutrophil recruitment.
  • Fig. S3. Positive hit compounds have no significant effects on total neutrophil number.
  • Fig. S4. Cryptotanshinone accelerates neutrophil apoptosis in vitro and overrides GM-CSF–induced survival.
  • Fig. S5. Inflammation resolution is delayed in the presence of dominant-active hif-1αb.
  • Fig. S6. Tanshinone IIA does not affect neutrophil reverse transmigration across endothelial monolayers in vitro.
  • Fig. S7. Tanshinone IIA does not alter cytokine production in stimulated monocytes.

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