Supplementary Materials

Supplementary Material for:

Cyclin A2 Induces Cardiac Regeneration After Myocardial Infarction Through Cytokinesis of Adult Cardiomyocytes

Scott D. Shapiro, Amaresh K. Ranjan, Yoshiaki Kawase, Richard K. Cheng, Rina J. Kara, Romit Bhattacharya, Gabriela Guzman-Martinez, Javier Sanz, Mario J. Garcia, Hina W. Chaudhry*

*Corresponding author. E-mail:

Published 19 February 2014, Sci. Transl. Med. 6, 224ra27 (2014)
DOI: 10.1126/scitranslmed.3007668

This PDF file includes:

  • Fig. S1. Cyclin A2 protein expression was determined at 6 weeks after injection of adenoviruses.
  • Fig. S2. MRI delayed enhancement studies exhibit a trend toward scar reduction in cyclin A2–treated animals.
  • Fig. S3. Measurement of cell size and number was performed by delineating cardiomyocyte cell borders.
  • Fig. S4. Cyclin A2 expression in cardiomyocyte nuclei was confirmed with immunofluorescence analysis in fixed cells.
  • Fig. S5. Immunofluorescence control experiments verify cardiomyocyte identity.
  • Table S1. Baseline studies of EDV and ESV as measured with 2D and 3D echocardiography exhibit no differences in baseline EF.
  • Table S2. EDV and ESV were measured with LV angiography, 2D and 3D echocardiography, and MRI.
  • Table S3. Cytokinetic indices of adult porcine cardiomyocytes were measured over 34 ROIs.
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). 2D echocardiography was used for EF assessment of porcine hearts.
  • Movie S2 (.mov format). 3D echocardiography was performed to measure EF of porcine hearts.
  • Movie S3 (.mov format). MRI was also used for EF determination of porcine hearts.
  • Movie S4 (.mov format). Rotational analysis of Z-stack image of mitotic nucleus in Fig. 3C.
  • Movie S5 (.mov format). Cytokinesis of adult porcine cardiomyocyte is captured by live cell imaging using time-lapse microscopy.

[Movies S1 to S5]