Supplementary Materials

Supplementary Material for:

Intramuscular Therapeutic Vaccination Targeting HPV16 Induces T Cell Responses That Localize in Mucosal Lesions

Leonel Maldonado, Jessica E. Teague, Matthew P. Morrow, Iveta Jotova, T. C. Wu, Chenguang Wang, Cindy Desmarais, Jean D. Boyer, Benjamin Tycko, Harlan S. Robins, Rachael A. Clark, Cornelia L. Trimble*

*Corresponding author. E-mail:

Published 29 January 2014, Sci. Transl. Med. 6, 221ra13 (2014)
DOI: 10.1126/scitranslmed.3007323

This PDF file includes:

  • Fig. S1. Intramuscular immunization with DNAE7 prime, followed by recombinant vaccinia (rVacE6E7) boost, induces HPV16 E6 and E7–specific TH1 immune responses in the peripheral blood.
  • Fig. S2. The intensity of lesional stromal CD8+ infiltrates increases significantly over a prospective 15-week window in vaccinated compared to unvaccinated subjects with HPV16+ CIN2/3 lesions.
  • Fig. S3. Gating strategy and representative isotype controls for the flow cytometry studies of cervical T cells.
  • Fig. S4. Frequencies of shared TCRs in tissue and blood.
  • Table S1. Severity of injection site reaction by cohort.
  • Table S2. Severity of AEs by system organ class and preferred term.
  • Table S3. mRNA expression of T cell–related genes in microdissected mucosal epithelium and stroma.

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