Supplementary Materials

Supplementary Material for:

Allelic-Dependent Expression of an Activating Fc Receptor on B Cells Enhances Humoral Immune Responses

Xinrui Li, Jianming Wu, Travis Ptacek, David T. Redden, Elizabeth E. Brown, Graciela S. Alarcón, Rosalind Ramsey-Goldman, Michelle A. Petri, John D. Reveille, Richard A. Kaslow, Robert P. Kimberly,* Jeffrey C. Edberg*

*Corresponding author. E-mail: rpk{at}uab.edu (R.P.K.); jedberg{at}uab.edu (J.C.E.)

Published 18 December 2013, Sci. Transl. Med. 5, 216ra175 (2013)
DOI: 10.1126/scitranslmed.3007097

This PDF file includes:

  • Fig. S1. Specificity of anti-Fcγ RIIc mAb 11B6 and anti-Fcγ RIIb mAb 3B6.
  • Fig. S2. Flow cytometric analysis of Fcγ RIIc and Fcγ RIIb expression in stable A20-IIA1.6 transductants.
  • Fig. S3. Absence of transgene expression on macrophages and T cells in TG mice.
  • Fig. S4. TG mice show normal proportions of B cell subsets.
  • Fig. S5. No difference in naïve IgM and IgG levels between TG and NTG mice.
  • Fig. S6. Enhanced B cell proliferation and activation in TG mice.
  • Fig. S7. Enhanced binding stability of the anti-TNP response in TG compared to NTG mice.
  • Fig. S8. Decreased frequency of FCGR2C-ORF in nonresponders in an anthrax vaccine trial.
  • Table S1. Demographics of the healthy volunteers participating in the CDC-sponsored NCT00119067 AVA study available for ancillary study on genetic correlates.
  • Table S2. Demographics of the PROFILE case-control study.
  • Table S3. List of primers.

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