Supplementary Materials

Supplementary Material for:

Pharmacological Inhibition of a MicroRNA Family in Nonhuman Primates by a Seed-Targeting 8-Mer AntimiR

Veerle Rottiers, Susanna Obad, Andreas Petri, Robert McGarrah, Marie W. Lindholm, Joshua C. Black, Sumita Sinha, Robin J. Goody, Matthew S. Lawrence, Andrew S. deLemos, Henrik F. Hansen, Steve Whittaker, Steve Henry, Rohn Brookes, Seyed Hani Najafi-Shoushtari, Raymond T. Chung, Johnathan R. Whetstine, Robert E. Gerszten, Sakari Kauppinen,* Anders M. Näär*

*Corresponding author. E-mail: naar@helix.mgh.harvard.edu (A.M.N.); sk@bio.aau.dk (S.K.)

Published 20 November 2013, Sci. Transl. Med. 5, 212ra162 (2013)
DOI: 10.1126/scitranslmed.3006840

This PDF file includes:

  • Fig. S1. ABCA1 mRNA in HepG2 cells and total cholesterol in mice after treatment with control oligonucleotides.
  • Fig. S2. Hepatic ABCA1 protein in African green monkeys after treatment with antimiR-33a, antimiR-33b, or antimiR-33a/b.
  • Fig. S3. Plasma glucose and insulin concentrations in vehicle- and antimiR-33– treated African green monkeys.
  • Fig. S4. Quantification of hepatic miR-33a and miR-33b in control African green monkeys.
  • Fig. S5. Plasma triglyceride concentrations in antimiR-33–treated African green monkeys.
  • Fig. S6. Clinical chemistry measurements in antimiR-33a/b– and vehicle-treated African green monkeys.
  • Table S1. Characteristics of the LNA-modified antimiRs used in this study.
  • Table S2 legend
  • Table S3. Weight of the African green monkeys at the start of the study.
  • Table S4. Predicted miR-33 targets with increased expression after antimiR-33a/b treatment.
  • Table S5. Pharmacokinetic properties of the 8-mer antimiR-33a/b oligonucleotide.
  • Table S6. Assessment of liver steatosis after treatment with the 8-mer LNA-modified antimiR-33a/b.
  • Table S7. GO term analysis of genes with changed expression after antimiR-33a/b treatment.

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Other Supplementary Material for this manuscript includes the following:

  • Table S2. Raw data and P values for data with a sample size of n < 20 (provided separately in an Excel file).

[Table S2]