Supplementary Materials

Supplementary Material for:

Differential Diagnosis of Azoospermia with Proteomic Biomarkers ECM1 and TEX101 Quantified in Seminal Plasma

Andrei P. Drabovich, Apostolos Dimitromanolakis, Punit Saraon, Antoninus Soosaipillai, Ihor Batruch, Brendan Mullen, Keith Jarvi, Eleftherios P. Diamandis*

*Corresponding author. E-mail: ediamandis@mtsinai.on.ca

Published 20 November 2013, Sci. Transl. Med. 5, 212ra160 (2013)
DOI: 10.1126/scitranslmed.3006260

This PDF file includes:

  • Fig. S1. Two-peptide SRM assay for biomarker candidates in SP.
  • Fig. S2. Calibration curves used to establish LODs and LOQs of SRM assays.
  • Fig. S3. LC-SRM chromatograms of TEX101 and ECM1 proteins in three patients’ SP samples.
  • Fig. S4. Heat map of log2-transformed mean-centered protein concentrations measured by SRM.
  • Fig. S5. TEX101, LDHC, PTGDS, and ECM1 in SP before and after vasectomy.
  • Fig. S6. TEX101 tissue specificity.
  • Table S1. Parameters of a multiplex SRM assay.
  • Table S2. TEX101 immunohistochemistry analysis.
    Table S3. Parameters of immuno-SRM assay for TEX101 protein.
  • Table S4. TEX101 protein sequence and proteotypic peptides analyzed by immuno-SRM assay.
  • Table S5. Power calculations.
  • Table S6. Summary of SP samples analyzed in the present study.
  • Table S7. Clinical information for 119 patients analyzed by SRM.
  • Table S8. Bioinformatic approach to verify the uniqueness of proteotypic peptides.
  • Table S9. Proteins and protein isoforms quantified by SRM assay.
  • Table S10. Single-nucleotide variants and posttranslational modifications that affect quantitative SRM data.

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