Supplementary Materials

Supplementary Material for:

Aldehyde Dehydrogenase Expression Drives Human Regulatory T Cell Resistance to Posttransplantation Cyclophosphamide

Christopher G. Kanakry, Sudipto Ganguly, Marianna Zahurak, Javier Bolaños-Meade, Christopher Thoburn, Brandy Perkins, Ephraim J. Fuchs, Richard J. Jones, Allan D. Hess, Leo Luznik*

*Corresponding author. E-mail: luznile@jhmi.edu

Published 13 November 2013, Sci. Transl. Med. 5, 211ra157 (2013)
DOI: 10.1126/scitranslmed.3006960

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Phenotypically identified naïve and effector Tregs are functionally suppressive.
  • Fig. S2. Phenotypically identified naïve and effector Tregs are demethylated within the TSDR.
  • Fig. S3. Impact of immunosuppressants on CD4+ T cell inflammatory cytokine expression in MLR.
  • Fig. S4. Additional characterization of CD4+Foxp3+ T cells in patients after alloBMT.
  • Fig. S5. Corticosteroids reduce total numbers of CD4+ T cells without affecting relative percentages.
  • Fig. S6. The LIVE/DEAD Fixable Aqua Viability Dye identifies nearly all nonviable cells in MLR.
  • Fig. S7. CD95 expression in CD4+ fractions.
  • Fig. S8. Foxp3+ fractions remain resistant to mafosfamide despite the posttreatment addition of CsA.
  • Fig. S9. Up-regulation of Foxp3 expression in MLR correlates with ALDH1A1 expression.
  • Fig. S10. Minimal ALDH3A1 expression in CD4+ T cells at baseline and in MLR.
  • Fig. S11. DEAB prevents Tcon activation in MLR.
  • Fig. S12. Aldefluor+CD4+ T cells are resistant to mafosfamide.

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