Supplementary Materials

Supplementary Material for:

Spherical Nucleic Acid Nanoparticle Conjugates as an RNAi-Based Therapy for Glioblastoma

Samuel A. Jensen, Emily S. Day, Caroline H. Ko, Lisa A. Hurley, Janina P. Luciano, Fotini M. Kouri, Timothy J. Merkel, Andrea J. Luthi, Pinal C. Patel, Joshua I. Cutler, Weston L. Daniel, Alexander W. Scott, Matthew W. Rotz, Thomas J. Meade, David A. Giljohann, Chad A. Mirkin,* Alexander H. Stegh*

*Corresponding author. E-mail: a-stegh@northwestern.edu (A.H.S.); chadnano@northwestern.edu (C.A.M.)

Published 30 October 2013, Sci. Transl. Med. 5, 209ra152 (2013)
DOI: 10.1126/scitranslmed.3006839

This PDF file includes:

  • Methods
  • Fig. S1. Bcl2L12 is a GBM oncogene.
  • Fig. S2. SNA uptake into murine astrocytes and muTNS depends on scavenger receptors.
  • Fig. S3. Synthesis scheme for SNAs.
  • Fig. S4. Evaluation of additional SNAs targeting Bcl2L12.
  • Fig. S5. Biodistribution of SNAs in glioma-bearing mice after systemic administration.
  • Fig. S6. The BBB/BTB is compromised in tumor-bearing mouse brains.
  • Fig. S7. Pharmacokinetics of SNAs.
  • Fig. S8. Biodistribution of SNAs in tumor-free mice.
  • Fig. S9. Histological evaluation of siL12-2-SNAs in major tissues.
  • Fig. S10. Histological analysis of huTNS-derived mouse tumors.
  • Table S1. Characterization of SNAs.
  • Table S2. Evaluation of serum cytokines in mice.
  • Table S3. Body weight and blood chemistry after systemic SNA administration to Sprague-Dawley rats.
  • Legend for movie S1
  • References (4042)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). 3D reconstruction of MR images after intracranial injection of Gd(III)-functionalized SNAs.

[Movie S1]