Supplementary Materials

Supplementary Material for:

Genetic and Environmental Determinants of Human NK Cell Diversity Revealed by Mass Cytometry

Amir Horowitz, Dara M. Strauss-Albee, Michael Leipold, Jessica Kubo, Neda Nemat-Gorgani, Ozge C. Dogan, Cornelia L. Dekker, Sally Mackey, Holden Maecker, Gary E. Swan, Mark M. Davis, Paul J. Norman, Lisbeth A. Guethlein, Manisha Desai, Peter Parham, Catherine A. Blish*

*Corresponding author. E-mail:

Published 23 October 2013, Sci. Transl. Med. 5, 208ra145 (2013)
DOI: 10.1126/scitranslmed.3006702

This PDF file includes:

  • Fig. S1. Validation of mass cytometry panel for phenotyping NK cells.
  • Fig. S2. Gating strategy for defining NK cells and ranges of frequencies of NK cells expressing each individual marker.
  • Fig. S3. KIR and HLA class I genotyping.
  • Fig. S4. Representative two-dimensional mass cytometry plots of NK cells from one representative donor (HNK-002) showing expression of all 28 NK cell receptors individually when expressed against CD56.
  • Fig. S5. SPADE trees showing distribution of cell lineage markers across unfractionated PBMC.
  • Fig. S6. Clustering analysis of NK cells from Twin-5a and Twin-5b using SPADE.
  • Fig. S7. Clustering analysis of NK cells from HNK-003 and HNK-007 using SPADE.
  • Table S1. Mass cytometry panel antibody information.
  • Table S2. Mass cytometry isotype control and LSR antibody panel information.

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