Supplementary Materials

Supplementary Material for:

Sulfate Metabolites Provide an Intracellular Pool for Resveratrol Generation and Induce Autophagy with Senescence

Ketan R. Patel, Catherine Andreadi, Robert G. Britton, Emma Horner-Glister, Ankur Karmokar, Stewart Sale, Victoria A. Brown, Dean E. Brenner, Rajinder Singh, William P. Steward, Andreas J. Gescher, Karen Brown*

*Corresponding author. E-mail: kb20@le.ac.uk

Published 2 October 2013, Sci. Transl. Med. 5, 205ra133 (2013)
DOI: 10.1126/scitranslmed.3005870

This PDF file includes:

  • Materials and Methods
  • Fig. S1. 1H nuclear magnetic resonance spectrum of resveratrol sulfate.
  • Fig. S2. Metabolite profile in mouse tissues after oral resveratrol sulfate.
  • Fig. S3. Metabolite pharmacokinetics in mice.
  • Fig. S4. Kinetics of resveratrol/metabolite formation in cell medium.
  • Fig. S5. Expression of transporter genes.
  • Fig. S6. Cell cycle and apoptosis analysis.
  • Fig. S7. Autophagy and senescence markers in HCEC cells.
  • Table S1. Levels of resveratrol and metabolites in human plasma.
  • Table S2. Comparison of accurately quantified and estimated colorectal tissue levels of resveratrol metabolites.
  • Table S3. Mouse pharmacokinetic parameters after resveratrol sulfate administration.
  • Table S4. Comparison of pharmacokinetic parameters for resveratrol and its metabolites.
  • Table S5. Intracellular concentrations in HT-29 cells.

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