Supplementary Materials

Supplementary Material for:

siRNA Screen for Genes That Affect Junín Virus Entry Uncovers Voltage-Gated Calcium Channels as a Therapeutic Target

Madakasira Lavanya, Christian D. Cuevas, Monica Thomas, Sara Cherry, Susan R. Ross*

*Corresponding author. E-mail: rosss@mail.med.upenn.edu

Published 25 September 2013, Sci. Transl. Med. 5, 204ra131 (2013)
DOI: 10.1126/scitranslmed.3006827

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Graph of Ζ scores showing infection levels and DAPI staining with the two sets of siRNAs used in the primary screen.
  • Fig. S2. Expression validation of the 10 genes in U2OS and 293T cells.
  • Fig. S3. Validation of knockdown of 10 genes by siRNAs.
  • Fig. S4. Effect of siRNA knockdown of 10 genes on Machupo and MLV pseudotype infection.
  • Fig. S5. TfR1 function is not altered by siRNA knockdown of 10 genes.
  • Fig. S6. TfR1 function is not altered by siRNAs targeting VGCC subunit genes or calcium channel blocker treatment.
  • Fig. S7. Expression of α1S and α2δ2 subunits after siRNA transfection targeting different VGCC subunits.
  • Fig. S8. IC50 values and MTT assays for verapamil, nifedipine, and gabapentin.
  • Fig. S9. Knockdown or inhibition of VGCCs decreases Lassa and LCMV pseudovirus infection.
  • Fig. S10. VGCC blockers inhibit an early stage of Junín pseudovirus infection.
  • Fig. S11. Candid 1 binds to VGCCs on mouse cells.
  • Fig. S12. VGCCs are important for Junín pseudovirus and Candid 1 infection of mouse cells.
  • Table S1. Genes identified in the siRNA screen as affecting Junín pseudovirus infection.
  • Table S2. Ζ scores of all VGCC subunit channels present in primary screen.
  • Table S3. Sequences of siRNAs used in secondary screen.
  • Table S4. Additional siRNAs used in infection assays.

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