Supplementary Materials

Supplementary Material for:

Generation of Effector Memory T Cell–Based Mucosal and Systemic Immunity with Pulmonary Nanoparticle Vaccination

Adrienne V. Li, James J. Moon, Wuhbet Abraham, Heikyung Suh, Jamal Elkhader, Michael A. Seidman, Minmin Yen, Eung-Jun Im, Maria H. Foley, Dan H. Barouch, Darrell J. Irvine*

*Corresponding author. E-mail: djirvine@mit.edu

Published 25 September 2013, Sci. Transl. Med. 5, 204ra130 (2013)
DOI: 10.1126/scitranslmed.3006516

This PDF file includes:

  • Materials and Methods
  • Fig. S1. ICMV nanoparticles adjuvanted with TLR agonists elicit CD8+ T cell responses after pulmonary vaccination.
  • Fig. S2. Phenotype of OVA+ APCs in lungs and mdLNs.
  • Fig. S3. Pulmonary vaccination elicits humoral immune responses in the blood and vaginal tract.
  • Fig. S4. OVA-tetramer+ T cells from reproductive tract and gut after pulmonary immunization.
  • Fig. S5. Pulmonary nanocapsule vaccination promotes TEM cell response in systemic and mucosal compartments.
  • Fig. S6. Immunization with AL11/PADRE peptide ICMVs induces effector memory–biased T cell responses.
  • Fig. S7. The role of TEM and TCM cells in ICMV immunization.
  • Fig. S8. Pulmonary nanocapsule vaccination does not induce weight loss in healthy animals after vaccination.
  • Fig. S9. Chemokine and cytokine levels in lungs, serum, and BAL after pulmonary nanocapsule vaccination.

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