Supplementary Materials

Supplementary Material for:

IL-29 Is Produced by TH17 Cells and Mediates the Cutaneous Antiviral Competence in Psoriasis

Kerstin Wolk,* Katrin Witte, Ellen Witte, Martin Raftery, Georgios Kokolakis, Sandra Philipp, Günther Schönrich, Katarzyna Warszawska, Stefan Kirsch, Susanna Prösch, Wolfram Sterry, Hans-Dieter Volk, Robert Sabat*

*Corresponding author. E-mail: (R.S.); (K. Wolk)

Published 25 September 2013, Sci. Transl. Med. 5, 204ra129 (2013)
DOI: 10.1126/scitranslmed.3006245

This PDF file includes:

  • Methods
  • Fig. S1. AVPs are expressed in lesional, but not in uninvolved, skin of psoriasis patients.
  • Fig. S2. Type I IFNs are absent in chronic psoriatic lesions and inhibit the production of psoriasis-relevant T cell cytokines.
  • Fig. S3. In psoriatic lesions, IL-29 levels selectively correlate with AVP levels.
  • Fig. S4. IL-29 increases AVP expression and antiviral defense in keratinocytes, whereas TH2 cytokines do not influence AVP expression.
  • Fig. S5. Psoriatic lesions show an elevated expression of IL-29 signaling elements.
  • Fig. S6. IFN-γ amplifies IL-29 effects.
  • Fig. S7. pDCs, myeloid cell populations, and keratinocytes show a limited IL-29 production capacity.
  • Fig. S8. In vitro–generated TH subsets show lineage-specific expression of cytokines and transcriptions factors.
  • Fig. S9. IL-29 production in TH17 cells is dependent on RORγt/RORα.
  • Table S1. Culture conditions for TH cell differentiation.
  • Table S2. Antibodies used for flow cytometry and immunohistochemistry/immunofluorescence.

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