Supplementary Materials

Supplementary Material for:

Angiopoietin-1 Guides Directional Angiogenesis Through Integrin αvβ5 Signaling for Recovery of Ischemic Retinopathy

Junyeop Lee, Kyung Eun Kim, Dong-Kyu Choi, Jeon Yeob Jang, Ji-Jung Jung, Hiroshi Kiyonari, Go Shioi, Woohyok Chang, Toshio Suda, Naoki Mochizuki, Yoshikazu Nakaoka, Issei Komuro, Ook-Joon Yoo,* Gou Young Koh*

*Corresponding authors. E-mail: (G.Y.K.); (O.-J.Y.)

Published 18 September 2013, Sci. Transl. Med. 5, 203ra127 (2013)
DOI: 10.1126/scitranslmed.3006666

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Ang1 is expressed in retinal pericytes and efficiently excised after tamoxifen treatment in Ang1fl/fl mouse.
  • Fig. S2. Ang1 suppresses the expression of Ang2 and VEGF-A in the retinas of OIR model.
  • Fig. S3. Ang1 suppresses the NVT and avascular areas in OIR model in a time- and dose-dependent manner.
  • Fig. S4. Combination of Ang1 and VEGF-Trap is more beneficial than single administration of VEGF-Trap in OIR model.
  • Fig. S5. Isolated retinal astrocytes were cultured, and their purity was confirmed using fluorescence-activated cell sorting analysis.
  • Fig. S6. Blood vessels, not astrocytes, express Tie2 in the retina during the postnatal period and in the OIR model.
  • Fig. S7. Ang1 directly binds integrins αv and β5 in primary cultured retinal astrocytes.
  • Fig. S8. FAK-AKT signaling pathway is involved in Ang1-induced fibronectin synthesis.
  • References (50, 51)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. (Microsoft Excel format). Raw data for graphs.
  • Table S2. (Microsoft Excel format). Primer sequences used for sqRT-PCR and quantitative real-time PCR.

[Tables S1 and S2]