Supplementary Materials

Supplementary Material for:

Down-Regulation of Autophagy-Related Protein 5 (ATG5) Contributes to the Pathogenesis of Early-Stage Cutaneous Melanoma

He Liu, Zhaoyue He, Thomas von Rütte, Shida Yousefi, Robert E. Hunger, Hans-Uwe Simon*

*Corresponding author. E-mail:

Published 11 September 2013, Sci. Transl. Med. 5, 202ra123 (2013)
DOI: 10.1126/scitranslmed.3005864

This PDF file includes:

  • Fig. S1. ATG5 mRNA expression is compared among different cancer cell lines.
  • Fig. S2. ATG5 is homogeneously expressed in most of the patients.
  • Fig. S3. ATG5 expression is quantified in melanoma patients according to their Clark levels.
  • Fig. S4. The expression of ATG5, Beclin 1, LC3, and p62 is detected in melanoma and nevus patients.
  • Fig. S5. ATG5 expression and autophagy are down-regulated in a subpopulation of melanoma cell lines.
  • Fig. S6. A putative CpG island in the ATG5 promoter is illustrated using the online analysis tool EMBL-EBI CpG plot.
  • Fig. S7. Starvation-induced autophagic flux is shown in ATG5-deficient A-2058 melanoma cells in the presence and absence of 5-aza-dC.
  • Fig. S8. Autophagosome formation is shown by LC3 and Autodot staining.
  • Fig. S9. Overexpression of ATG5 induces apoptosis in melanoma cells.
  • Fig. S10. Treatment of ATG5-deficient melanoma cells with 5-aza-dC inhibits proliferation and induces senescence.
  • Fig. S11. Successful gene transfer in isolated melanocytes is demonstrated by immunoblotting.
  • Fig. S12. Knockdown of ATG5 increases proliferation of melanocytes expressing BRAF or HRAS oncogenes.
  • Fig. S13. Knockdown of ATG5 decreases autophagy in melanocytes expressing BRAF or HRAS oncogenes.
  • Fig. S14. Knockdown of ATG5 decreases senescence in melanocytes expressing BRAF or HRAS oncogenes.
  • Table S1. Information about the patients included in this study.
  • Table S2. Information about the patients selected for LC3 immunostaining analysis.
  • Table S3. ATG5 promoter methylation and ATG5 mRNA expression in melanoma cell lines.

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