Supplementary Materials

Supplementary Material for:

Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy

Minbiao Ji, Daniel A. Orringer, Christian W. Freudiger, Shakti Ramkissoon, Xiaohui Liu, Darryl Lau, Alexandra J. Golby, Isaiah Norton, Marika Hayashi, Nathalie Y. R. Agar, Geoffrey S. Young, Cathie Spino, Sandro Santagata, Sandra Camelo-Piragua, Keith L. Ligon, Oren Sagher, X. Sunney Xie*

*Corresponding author. E-mail: xie@chemistry.harvard.edu

Published 4 September 2013, Sci. Transl. Med. 5, 201ra119 (2013)
DOI: 10.1126/scitranslmed.3005954

This PDF file includes:

  • Methods
  • Fig. S1. Raman spectra of brain tissue, lipids, and proteins.
  • Fig. S2. Representative infiltrating glioma SRS FOVs correctly classified by neuropathologists.
  • Fig. S3. Comparison between transmission and epi-SRS images.
  • Fig. S4. Comparison of SRS images in fresh and frozen tissues.
  • Fig. S5. SRS images of various mouse models of human brain tumors.
  • Fig. S6. Power scaling of SRS microscopy.
  • Fig. S7. Additional in vivo SRS images of GBM xenografts.
  • Fig. S8. Coronal H&E staining after in vivo imaging.
  • Table S1. Compiled contingency table summarizing survey results.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Three-dimensional depth profile of brain tumor margin.

[Movie S1]