Supplementary Materials

Supplementary Material for:

Up-Regulation of PD-L1, IDO, and Tregs in the Melanoma Tumor Microenvironment Is Driven by CD8+ T Cells

Stefani Spranger, Robbert M. Spaapen, Yuanyuan Zha, Jason Williams, Yuru Meng, Thanh T. Ha, Thomas F. Gajewski*

*Corresponding author. E-mail:

Published 28 August 2013, Sci. Transl. Med. 5, 200ra116 (2013)
DOI: 10.1126/scitranslmed.3006504

This PDF file includes:

  • Fig. S1. Costaining of CD4 and FoxP3 in human melanoma sections.
  • Fig. S2. Expression level of IDO, PD-L1, and MHC I/II in B16 tumors in vitro and in vivo.
  • Fig. S3. Lack of Treg conversion from CD25-depleted T cells.
  • Fig. S4. Characterization of TILs compared to in vitro–primed CD8 T cells.
  • Fig. S5. CCR4 expression on human CD4 T cells and Tregs.
  • Fig. S6. Suppressive functional capacity of human CD25hi Tregs.

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