Supplementary Materials

Supplementary Material for:

Vaccine-Induced Anti-HA2 Antibodies Promote Virus Fusion and Enhance Influenza Virus Respiratory Disease

Surender Khurana, Crystal L. Loving, Jody Manischewitz, Lisa R. King, Phillip C. Gauger, Jamie Henningson, Amy L. Vincent,* Hana Golding*

*Corresponding author. E-mail: (H.G.); (A.L.V.)

Published 28 August 2013, Sci. Transl. Med. 5, 200ra114 (2013)
DOI: 10.1126/scitranslmed.3006366

This PDF file includes:

  • Fig. S1. Pairwise alignment of the HA amino acid sequence of pH1N1 (A/California/04/2009) and H1N2 (A/Swine/Minnesota/02011/2008).
  • Fig. S2. Phylogenetic tree of HA proteins from human and swine H1 viruses.
  • Fig. S3. Anti-HA2 antibodies can compete with pH1N1-neutralizing antibodies.
  • Fig. S4. WIV-H1N2 postvaccination serum antibody binding to N-terminal HA1 domain is mediated by IgM isotype antibody.
  • Fig. S5. Binding of pH1N1 virus to sialic acid receptor in the absence and presence of WIV-H1N2 sera in SPR assay.
  • Fig. S6. No enhancement of pH1N1 virus–mediated hemagglutination in the presence of WIV-H1N2 serum antibodies.

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