Supplementary Materials

Supplementary Material for:

The Identification and Characterization of Breast Cancer CTCs Competent for Brain Metastasis

Lixin Zhang, Lon D. Ridgway, Michael D. Wetzel, Jason Ngo, Wei Yin, Disha Kumar, Jerry C. Goodman, Morris D. Groves, Dario Marchetti*

*Corresponding author. E-mail:

Published 10 April 2013, Sci. Transl. Med. 5, 180ra48 (2013)
DOI: 10.1126/scitranslmed.3005109

This PDF file includes:

  • Methods
  • Fig. S1. Morphology of isolated CTCs.
  • Fig. S2. CTCs do not express the MSC marker triplet.
  • Fig. S3. The BMSM CTC signature is maintained in long-term CTC culture.
  • Fig. S4. IF analyses of parental CTCs.
  • Fig. S5. Gating parameters for FACS sorting of BMSM CTCs.
  • Fig. S6. IF analysis of BMSM CTCs.
  • Fig. S7. BMBC morphology and EGFR IHC following BMSM CTC-1 xenograft.
  • Fig. S8. BMSM protein marker expression in BMSM CTC–induced brain metastasis.
  • Table S1. FACS-captured CACCs, cell survival, and in vitro growth.
  • Table S2. Thermodynamically matched primers for RT-PCR analysis.
  • Table S3. CellSearch analysis of FACS-sorted EpCAM CTC lines.
  • Table S4. STR DNA fingerprinting profiles of parental CTC lines.
  • Table S5. Detection of cancer-associated mutations in parental CTC lines.
  • Table S6. Isolation and sorting of PBMCs from breast cancer patients.
  • Table S7. Tumor burden of BMSM CTC–induced brain metastases.

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