Supplementary Materials

Supplementary Material for:

Induction of ICOS+CXCR3+CXCR5+ TH Cells Correlates with Antibody Responses to Influenza Vaccination

Salah-Eddine Bentebibel, Santiago Lopez, Gerlinde Obermoser, Nathalie Schmitt, Cynthia Mueller, Carson Harrod, Emilio Flano, Asuncion Mejias, Randy A. Albrecht, Derek Blankenship, Hui Xu, Virginia Pascual, Jacques Banchereau, Adolfo Garcia-Sastre, Anna Karolina Palucka, Octavio Ramilo,* Hideki Ueno*

*Corresponding author. E-mail: Octavio.Ramilo@nationwidechildrens.org (O.R.); hidekiu@baylorhealth.edu (H.U.)

Published 13 March 2013, Sci. Transl. Med. 5, 176ra32 (2013)
DOI: 10.1126/scitranslmed.3005191

This PDF file includes:

  • Materials and Methods
  • Fig. S1. CD4+ and CXCR5+CD4+ T cells did not change after influenza vaccination.
  • Fig. S2. Blood CXCR3+CCR6+ cell population within CXCR5+CD4+ T cells did not change by influenza vaccination.
  • Fig. S3. HI and VN titers in adult cohort.
  • Fig. S4. HI and VN titers in children cohort.
  • Fig. S5. Positive correlation between the increase of ICOS+CXCR3+CXCR5+CD4+ T cells and the increase of plasmablasts in the children cohort, when the four donors who showed >5 plasmablasts/μl of blood at baseline were removed from the analysis.
  • Fig. S6. Positive correlation between the increase of ICOS+CXCR3+CXCR5+CD4+ T cells and the increased antibody titers against each viral strain in the vaccine.
  • Fig. S7. Positive correlation between the increase of ICOS+CXCR3+CXCR5+CD4+ T cells and the increased antibody titers against H1N1 2009 in the vaccine.
  • Fig. S8. CD154 expression by ICOS+CXCR5+CD4+ T cells was independent of soluble factors secreted during the stimulation.
  • Fig. S9. Induced ICOS+CXCR5+CD4+ T cells did not express CD154 upon stimulation with CMV peptides.
  • Fig. S10. ICOSCD4+ T cells did not express ICOS within 6 hours of activation.
  • Fig. S11. Induced ICOS+CXCR3+CXCR5+CD4+ T cells express multiple cytokines upon stimulation with heat-killed PR8 virus.
  • Fig. S12. Cytokine profiles of ICOSCXCR5CD4+ T cells upon stimulation with Fluzone.
  • Fig. S13. ICOS+CXCR3+CXCR5+CD4+ T cells help memory B cells in a manner dependent on IL-10 and IL-21.
  • Table S1. Absolute cell number of ICOS+CXCR3++CXCR5+CD4+ T cells in blood at day 7 correlates with antibody responses.
  • Table S2. Correlations between the increase of ICOS+CXCR3+CXCR5+CD4+ T cells and the increased antibody titers against each viral strain in the vaccines.

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