Supplementary Materials

Supplementary Materials for:

Instructive Nanofiber Scaffolds with VEGF Create a Microenvironment for Arteriogenesis and Cardiac Repair

Yi-Dong Lin, Chwan-Yau Luo, Yu-Ning Hu, Ming-Long Yeh, Ying-Chang Hsueh, Min-Yao Chang, Da-Ching Tsai, Jieh-Neng Wang, Ming-Jer Tang, Erika I. H. Wei, Matthew L. Springer, Patrick C. H. Hsieh*

*To whom correspondence should be addressed. E-mail: phsieh{at}mail.ncku.edu.tw

Published 8 August 2012, Sci. Transl. Med. 4, 146ra109 (2012)
DOI: 10.1126/scitranslmed.3003841

This PDF file includes:

  • Methods
  • Fig. S1. Human VEGF acts on rat endothelial cells in a dose-dependent manner.
  • Fig. S2. Peptide NFs exhibit a high binding capacity and a stable release kinetics profile of VEGF in vitro.
  • Fig. S3. NF/VEGF retards pathological remodeling at 28 days after MI.
  • Fig. S4. NF/VEGF and high-dose VEGF improve the peri-infarct capillary density at 28 days after MI.
  • Fig. S5. FluoSpheres estimate vessel leakage in vital organs at day 0.
  • Fig. S6. Representative flow cytometry–like scattergram of Ki67- and α-SMA– positive cells under various treatments and time points.
  • Fig. S7. FluoSpheres estimate vessel leakage in vital organs at day 7.
  • Fig. S8. Macrophage infiltration is attenuated by NF and/or VEGF injection after infarction.
  • Fig. S9. Immunostaining of cardiomyocyte-like small cells at the border zone from each treatment group at 28 days after MI in rats.
  • Fig. S10. Background signals from erythrocytes, but not GFP/cTnI+ cardiomyocyte-like cells, were detected in the negative control of staining.
  • Fig. S11. Cross sections at the level of papillary muscle insertion of left ventricle from each pig group.
  • Fig. S12. Capillaries at the border zone from each treatment group at 28 days after MI in pigs.
  • Fig. S13. Arterioles and arteries at the border zone from each treatment group at 28 days after MI in pigs.
  • Table S1. Hemodynamic parameters in pigs at 28 days after MI.

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