Supplementary Materials

Supplementary Material for:

Netting Neutrophils Are Major Inducers of Type I IFN Production in Pediatric Systemic Lupus Erythematosus

Gina S. Garcia-Romo, Simone Caielli, Barbara Vega, John Connolly, Florence Allantaz, Zhaohui Xu, Marilynn Punaro, Jeanine Baisch, Cristiana Guiducci, Robert L. Coffman, Franck J. Barrat, Jacques Banchereau, Virginia Pascual*

*To whom correspondence should be addressed. E-mail: virginip{at}baylorhealth.edu

Published 9 March 2011, Sci. Transl. Med. 3, 73ra20 (2011)
DOI: 10.1126/scitranslmed.3001201

This PDF file includes:

  • Fig. S1. IFN-α does not increase healthy neutrophil apoptosis.
  • Fig. S2. SLE serum or type I IFN reproduces many of the transcriptional alterations of SLE neutrophils.
  • Fig. S3. SLE serum and IFN-α up-regulate TLR7 expression in healthy neutrophils.
  • Fig. S4. Healthy and SLE neutrophils display different susceptibility to anti-RNP antibody–induced death as measured by the TUNEL assay.
  • Fig. S5. Elastase does not colocalize with LL37 and HMGB1 within NETs.
  • Fig. S6. Apoptotic and necrotic neutrophils fail to activate pDCs.
  • Fig. S7. SLE NETs induce pDC activation and survival.
  • Fig. S8. SLE NET activation of pDCs is independent of FcγRIIa.
  • Fig. S9. Release of extracellular DNA is specific to NETosis-associated death.
  • Fig. S10. IFN-α and anti-RNP antibody–induced NETs from healthy donors activate pDCs in a DNA-dependent manner.
  • Table S1. Demographic characteristics and disease activity of the SLE patients whose neutrophils were used for gene expression profiling.
  • Table S2. Demographic characteristics and disease activity of the SLE patients whose sera were used to culture healthy neutrophils in vitro for gene expression profiling.
  • Table S3. Characteristics of the SLE patients whose neutrophils were activated in vitro.
  • Table S4. Transcripts differentially expressed in freshly isolated SLE neutrophils.
  • Table S5. Transcripts differentially expressed in healthy neutrophils exposed to SLE sera in vitro.
  • Table S6. Transcripts differentially expressed in healthy neutrophils exposed to recombinant IFN-α2 in vitro.
  • Table S7. Corticosteroid therapy does not have an effect on the capacity of SLE neutrophils to undergo NETosis upon exposure to anti-RNP antibodies.

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