Supplementary Material

Supplementary Material for:

Isolation and Characterization of Circulating Tumor Cells from Patients with Localized and Metastatic Prostate Cancer

Shannon L. Stott, Richard J. Lee, Sunitha Nagrath, Min Yu, David T. Miyamoto, Lindsey Ulkus, Elizabeth J. Inserra, Matthew Ulman, Simeon Springer, Zev Nakamura, Alessandra L. Moore, Dina I. Tsukrov, Maria E. Kempner, Douglas M. Dahl, Chin-Lee Wu, A. John Iafrate, Matthew R. Smith, Ronald G. Tompkins, Lecia V. Sequist, Mehmet Toner, Daniel A. Haber,* Shyamala Maheswaran

*To whom correspondence should be addressed. E-mail: Haber{at}helix.mgh.harvard.edu

Published 31 March 2010, Sci. Transl. Med. 2, 25ra23 (2010)
DOI: 10.1126/scitranslmed.3000403

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of antibodies for detection of PSA in CTCs.
  • Fig. S2. Enumeration of PSA-positive CTCs in patients with prostate cancer and correlation with CK-positive CTCs.
  • Fig. S3. Serial monitoring of CTCs in additional metastatic prostate cancer patients receiving ADT.
  • Fig. S4. Serial monitoring of CTCs in metastatic prostate cancer patients receiving radiation or experimental chemotherapy regimens.
  • Fig. S5. Flow chart representing the image processing stream for each individual channel.
  • Table S1. Clinical characteristics and PSA-positive CTC counts for all metastatic patients presented in Fig. 2B.

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