Supplementary Material

Supplementary Material for:

Measurement and Clinical Monitoring of Human Lymphocyte Clonality by Massively Parallel VDJ Pyrosequencing

Scott D. Boyd, Eleanor L. Marshall, Jason D. Merker, Jay M. Maniar, Lyndon N. Zhang, Bita Sahaf, Carol D. Jones, Birgitte B. Simen, Bozena Hanczaruk, Khoa D. Nguyen, Kari C. Nadeau, Michael Egholm, David B. Miklos, James L. Zehnder, Andrew Z. Fire*

*To whom correspondence should be addressed. E-mail: afire{at}

Published 23 December 2009, Sci. Transl. Med. 1, 12ra23 (2009)
DOI: 10.1126/scitranslmed.3000540

This PDF file includes:

  • Materials and Methods
  • Fig. S1. V-D-J plots of healthy peripheral blood and lymphoid malignancies.
  • Fig. S2. Sequence complexity of healthy donor blood specimens.
  • Table S1. Sequencing primers.
  • Table S2. Patient specimens used in experiment 2.
  • Table S3. Number of sequences determined per specimen.
  • Table S4. Sequences found in more than one replicate from healthy donor blood samples.
  • References

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