A method of high-throughput functional evaluation of EGFR gene variants of unknown significance in cancer

Shinji Kohsaka; Masaaki Nagano; Toshihide Ueno; Yoshiyuki Suehara; Takuo Hayashi; Naoko Shimada; Kazuhisa Takahashi; Kenji Suzuki; Kazuya Takamochi; Fumiyuki Takahashi; Hiroyuki Mano

doi:10.1126/scitranslmed.aan6566

Research ResourcesCancer

A method of high-throughput functional evaluation of EGFR gene variants of unknown significance in cancer

View ORCID ProfileShinji Kohsaka¹,*,†,
Masaaki Nagano²,³,†,
View ORCID ProfileToshihide Ueno²,
Yoshiyuki Suehara⁴,
Takuo Hayashi⁵,
Naoko Shimada⁶,
Kazuhisa Takahashi⁶,
Kenji Suzuki⁷,
Kazuya Takamochi⁷,
Fumiyuki Takahashi⁶ and
View ORCID ProfileHiroyuki Mano²,⁸,*

¹Department of Medical Genomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
²Department of Cellular Signaling, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
³Department of Thoracic Surgery, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
⁴Department of Orthopedic Surgery, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.
⁵Department of Human Pathology, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.
⁶Department of Respiratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.
⁷Department of General Thoracic Surgery, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.
⁸National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan.

↵*Corresponding author. Email: kohsakas{at}m.u-tokyo.ac.jp (S.K.); hmano{at}m.u-tokyo.ac.jp (H.M.)

↵† These authors contributed equally to this work.

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Science Translational Medicine 15 Nov 2017:
Vol. 9, Issue 416, eaan6566
DOI: 10.1126/scitranslmed.aan6566

Shinji Kohsaka

1Department of Medical Genomics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

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ORCID record for Shinji Kohsaka
For correspondence: kohsakas@m.u-tokyo.ac.jp hmano@m.u-tokyo.ac.jp

Masaaki Nagano

2Department of Cellular Signaling, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

3Department of Thoracic Surgery, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

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Toshihide Ueno

2Department of Cellular Signaling, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

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ORCID record for Toshihide Ueno

Yoshiyuki Suehara

4Department of Orthopedic Surgery, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.

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Takuo Hayashi

5Department of Human Pathology, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.

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Naoko Shimada

6Department of Respiratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.

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Kazuhisa Takahashi

6Department of Respiratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.

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Kenji Suzuki

7Department of General Thoracic Surgery, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.

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Kazuya Takamochi

7Department of General Thoracic Surgery, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.

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Fumiyuki Takahashi

6Department of Respiratory Medicine, Graduate School of Medicine, Juntendo University, Bunkyo-ku, Tokyo 113-8431, Japan.

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Hiroyuki Mano

2Department of Cellular Signaling, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

8National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan.

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For correspondence: kohsakas@m.u-tokyo.ac.jp hmano@m.u-tokyo.ac.jp

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A method of high-throughput functional evaluation of EGFR gene variants of unknown significance in cancer

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