Research ArticleInfectious Disease

Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum

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Science Translational Medicine  27 Sep 2017:
Vol. 9, Issue 409, eaan1589
DOI: 10.1126/scitranslmed.aan1589

Distinguishing dengue from Zika

More than mere summer pests, mosquitoes can transmit viruses, such as dengue and Zika. Diagnosing infections of these related flaviviruses can be difficult because of cross-reactivity in diagnostic tests. Bosch et al. developed monoclonal antibodies to detect viral nonstructural 1 (NS1) protein antigens specific to dengue and Zika. Incorporating the antibodies into an immunochromatography format yielded a rapid diagnostic assay that produces a visual readout in the presence of NS1. The assay identified the four dengue serotypes and Zika viral infections without cross-reaction when testing human serum samples from endemic areas in Central and South America and India. This approach could be useful for developing rapid diagnostics for other emerging pathogens.

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