Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum

Irene Bosch; Helena de Puig; Megan Hiley; Marc Carré-Camps; Federico Perdomo-Celis; Carlos F. Narváez; Doris M. Salgado; Dewahar Senthoor; Madeline O’Grady; Elizabeth Phillips; Ann Durbin; Diana Fandos; Hikaru Miyazaki; Chun-Wan Yen; Margarita Gélvez-Ramírez; Rajas V. Warke; Lucas S. Ribeiro; Mauro M. Teixeira; Roque P. Almeida; José E. Muñóz-Medina; Juan E. Ludert; Mauricio L. Nogueira; Tatiana E. Colombo; Ana C. B. Terzian; Patricia T. Bozza; Andrea S. Calheiros; Yasmine R. Vieira; Giselle Barbosa-Lima; Alexandre Vizzoni; José Cerbino-Neto; Fernando A. Bozza; Thiago M. L. Souza; Monique R. O. Trugilho; Ana M. B. de Filippis; Patricia C. de Sequeira; Ernesto T. A. Marques; Tereza Magalhaes; Francisco J. Díaz; Berta N. Restrepo; Katerine Marín; Salim Mattar; Daniel Olson; Edwin J. Asturias; Mark Lucera; Mohit Singla; Guruprasad R. Medigeshi; Norma de Bosch; Justina Tam; Jose Gómez-Márquez; Charles Clavet; Luis Villar; Kimberly Hamad-Schifferli; Lee Gehrke

doi:10.1126/scitranslmed.aan1589

Research ArticleInfectious Disease

Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum

View ORCID ProfileIrene Bosch¹,²,*,
View ORCID ProfileHelena de Puig¹,³,*,
Megan Hiley¹,
View ORCID ProfileMarc Carré-Camps¹,⁴,
View ORCID ProfileFederico Perdomo-Celis⁵,
Carlos F. Narváez⁵,
Doris M. Salgado⁵,
View ORCID ProfileDewahar Senthoor¹,
Madeline O’Grady¹,
Elizabeth Phillips¹,
View ORCID ProfileAnn Durbin¹,⁶,
Diana Fandos¹,⁴,
Hikaru Miyazaki¹,
Chun-Wan Yen¹,
View ORCID ProfileMargarita Gélvez-Ramírez⁷,
Rajas V. Warke⁸,
View ORCID ProfileLucas S. Ribeiro⁹,
View ORCID ProfileMauro M. Teixeira⁹,
View ORCID ProfileRoque P. Almeida¹⁰,
View ORCID ProfileJosé E. Muñóz-Medina¹¹,
Juan E. Ludert¹²,
Mauricio L. Nogueira¹³,
Tatiana E. Colombo¹³,
Ana C. B. Terzian¹³,
View ORCID ProfilePatricia T. Bozza¹⁴,
Andrea S. Calheiros¹⁴,
Yasmine R. Vieira¹⁵,
Giselle Barbosa-Lima¹⁵,
Alexandre Vizzoni¹⁵,
José Cerbino-Neto¹⁵,
Fernando A. Bozza¹⁵,¹⁶,
Thiago M. L. Souza¹⁴,¹⁷,
View ORCID ProfileMonique R. O. Trugilho¹⁸,
Ana M. B. de Filippis¹⁹,
View ORCID ProfilePatricia C. de Sequeira¹⁹,
View ORCID ProfileErnesto T. A. Marques²⁰,²¹,
Tereza Magalhaes²⁰,²²,
View ORCID ProfileFrancisco J. Díaz²³,
Berta N. Restrepo²⁴,
Katerine Marín²⁴,
View ORCID ProfileSalim Mattar²⁵,
Daniel Olson²⁶,
Edwin J. Asturias²⁶,
Mark Lucera²⁷,
Mohit Singla²⁸,
View ORCID ProfileGuruprasad R. Medigeshi²⁹,
Norma de Bosch³⁰,
View ORCID ProfileJustina Tam¹,³¹,
View ORCID ProfileJose Gómez-Márquez¹,
Charles Clavet³¹,
View ORCID ProfileLuis Villar⁷,
View ORCID ProfileKimberly Hamad-Schifferli³,³²,† and
View ORCID ProfileLee Gehrke¹,³³,†

¹Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
²Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
³Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
⁴Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
⁵Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia.
⁶Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA.
⁷Universidad Industrial de Santander and AEDES Program (Alianza para el desarrollo de estrategias que disminuyan el impacto de enfermedades transmitidas por Aedes como resultado del estudio de sus endemias y epidemias), Bucaramanga, Santander, Colombia.
⁸HiMedia Laboratories Pvt. Ltd., Mumbai, India.
⁹Immunopharmacology Group, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Belo Horizonte, Brazil.
¹⁰Departamento de Medicina Interna e Patologia, Hospital Universitário/Empresa Brasileira de Serviços Hospitalares (EBSERH), Universidade Federal de Sergipe, Aracaju, Brazil.
¹¹Laboratorio Central de Epidemiología, Instituto Mexicano del Seguro Social, Avenida Jacarandas S/N, Esquina Circuito Interior, Colonia La Raza Del Azcapotzalco, Código Postal 02990 México D.F., México.
¹²Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, México.
¹³Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, Brazil.
¹⁴Immunopharmacology Laboratory, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.
¹⁵National Institute of Infectious Disease Evandro Chagas, FIOCRUZ, Rio de Janeiro, Brazil.
¹⁶D’Or Institute of Research and Education (IDOR), Rio de Janeiro, Brazil.
¹⁷National Institute for Science and Technology on Innovation on Neglected Diseases (INCT/IDN), Center for Technological Development in Health (CDTS), FIOCRUZ, Rio de Janeiro, Brazil.
¹⁸Toxinology Laboratory and Center for Technological Development in Health (CDTS), FIOCRUZ, Rio de Janeiro, Brazil.
¹⁹Flavivirus Laboratory, FIOCRUZ, Rio de Janeiro, Brazil.
²⁰Aggeu Magalhães Research Center, FIOCRUZ, Pernambuco, Recife, Brazil.
²¹Department of Infectious Disease and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
²²Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
²³Immunovirology Group, School of Medicine, University of Antioquia, Medellín, Colombia.
²⁴Instituto Colombiano de Medicina Tropical (ICMT), Universidad CES, Sabaneta, Antioquia, Colombia.
²⁵Universidad de Córdoba, Montería, Córdoba, Colombia.
²⁶Division of Infectious Diseases, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.
²⁷Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO 80045, USA.
²⁸Department of Paediatrics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
²⁹Translational Health Science and Technology Institute, Faridabad, India.
³⁰Universidad Central de Venezuela, Caracas, Venezuela.
³¹Winchester Engineering Analytical Center (WEAC), Winchester, MA 01890, USA.
³²Department of Engineering, University of Massachusetts Boston, Boston, MA 02125, USA.
³³Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.

↵†Corresponding author. Email: kim.hamad{at}umb.edu (K.H.-S.); lgehrke{at}mit.edu (L.G.)

↵* These authors contributed equally to this work.

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Science Translational Medicine 27 Sep 2017:
Vol. 9, Issue 409, eaan1589
DOI: 10.1126/scitranslmed.aan1589

Irene Bosch

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

2Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.

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Helena de Puig

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

3Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Megan Hiley

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Marc Carré-Camps

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.

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Federico Perdomo-Celis

5Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia.

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Carlos F. Narváez

5Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia.

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Doris M. Salgado

5Programa de Medicina, Facultad de Salud, Universidad Surcolombiana, Neiva, Colombia.

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Dewahar Senthoor

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Madeline O’Grady

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Elizabeth Phillips

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Ann Durbin

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

6Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA.

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Diana Fandos

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.

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Hikaru Miyazaki

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Chun-Wan Yen

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Margarita Gélvez-Ramírez

7Universidad Industrial de Santander and AEDES Program (Alianza para el desarrollo de estrategias que disminuyan el impacto de enfermedades transmitidas por Aedes como resultado del estudio de sus endemias y epidemias), Bucaramanga, Santander, Colombia.

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Rajas V. Warke

8HiMedia Laboratories Pvt. Ltd., Mumbai, India.

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Lucas S. Ribeiro

9Immunopharmacology Group, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Belo Horizonte, Brazil.

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Mauro M. Teixeira

9Immunopharmacology Group, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Belo Horizonte, Brazil.

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Roque P. Almeida

10Departamento de Medicina Interna e Patologia, Hospital Universitário/Empresa Brasileira de Serviços Hospitalares (EBSERH), Universidade Federal de Sergipe, Aracaju, Brazil.

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José E. Muñóz-Medina

11Laboratorio Central de Epidemiología, Instituto Mexicano del Seguro Social, Avenida Jacarandas S/N, Esquina Circuito Interior, Colonia La Raza Del Azcapotzalco, Código Postal 02990 México D.F., México.

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Juan E. Ludert

12Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, México.

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Mauricio L. Nogueira

13Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, Brazil.

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Tatiana E. Colombo

13Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, Brazil.

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Ana C. B. Terzian

13Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, Brazil.

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Patricia T. Bozza

14Immunopharmacology Laboratory, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.

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Andrea S. Calheiros

14Immunopharmacology Laboratory, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.

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Yasmine R. Vieira

15National Institute of Infectious Disease Evandro Chagas, FIOCRUZ, Rio de Janeiro, Brazil.

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Giselle Barbosa-Lima

15National Institute of Infectious Disease Evandro Chagas, FIOCRUZ, Rio de Janeiro, Brazil.

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Alexandre Vizzoni

15National Institute of Infectious Disease Evandro Chagas, FIOCRUZ, Rio de Janeiro, Brazil.

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José Cerbino-Neto

15National Institute of Infectious Disease Evandro Chagas, FIOCRUZ, Rio de Janeiro, Brazil.

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Fernando A. Bozza

15National Institute of Infectious Disease Evandro Chagas, FIOCRUZ, Rio de Janeiro, Brazil.

16D’Or Institute of Research and Education (IDOR), Rio de Janeiro, Brazil.

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Thiago M. L. Souza

14Immunopharmacology Laboratory, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.

17National Institute for Science and Technology on Innovation on Neglected Diseases (INCT/IDN), Center for Technological Development in Health (CDTS), FIOCRUZ, Rio de Janeiro, Brazil.

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Monique R. O. Trugilho

18Toxinology Laboratory and Center for Technological Development in Health (CDTS), FIOCRUZ, Rio de Janeiro, Brazil.

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Ana M. B. de Filippis

19Flavivirus Laboratory, FIOCRUZ, Rio de Janeiro, Brazil.

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Patricia C. de Sequeira

19Flavivirus Laboratory, FIOCRUZ, Rio de Janeiro, Brazil.

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Ernesto T. A. Marques

20Aggeu Magalhães Research Center, FIOCRUZ, Pernambuco, Recife, Brazil.

21Department of Infectious Disease and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.

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Tereza Magalhaes

20Aggeu Magalhães Research Center, FIOCRUZ, Pernambuco, Recife, Brazil.

22Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.

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Francisco J. Díaz

23Immunovirology Group, School of Medicine, University of Antioquia, Medellín, Colombia.

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Berta N. Restrepo

24Instituto Colombiano de Medicina Tropical (ICMT), Universidad CES, Sabaneta, Antioquia, Colombia.

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Katerine Marín

24Instituto Colombiano de Medicina Tropical (ICMT), Universidad CES, Sabaneta, Antioquia, Colombia.

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Salim Mattar

25Universidad de Córdoba, Montería, Córdoba, Colombia.

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Daniel Olson

26Division of Infectious Diseases, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.

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Edwin J. Asturias

26Division of Infectious Diseases, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.

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Mark Lucera

27Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO 80045, USA.

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Mohit Singla

28Department of Paediatrics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

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Guruprasad R. Medigeshi

29Translational Health Science and Technology Institute, Faridabad, India.

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Norma de Bosch

30Universidad Central de Venezuela, Caracas, Venezuela.

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Justina Tam

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

31Winchester Engineering Analytical Center (WEAC), Winchester, MA 01890, USA.

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Jose Gómez-Márquez

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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Charles Clavet

31Winchester Engineering Analytical Center (WEAC), Winchester, MA 01890, USA.

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Luis Villar

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Kimberly Hamad-Schifferli

3Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

32Department of Engineering, University of Massachusetts Boston, Boston, MA 02125, USA.

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For correspondence: kim.hamad@umb.edu lgehrke@mit.edu

Lee Gehrke

1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

33Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.

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For correspondence: kim.hamad@umb.edu lgehrke@mit.edu

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Distinguishing dengue from Zika

More than mere summer pests, mosquitoes can transmit viruses, such as dengue and Zika. Diagnosing infections of these related flaviviruses can be difficult because of cross-reactivity in diagnostic tests. Bosch et al. developed monoclonal antibodies to detect viral nonstructural 1 (NS1) protein antigens specific to dengue and Zika. Incorporating the antibodies into an immunochromatography format yielded a rapid diagnostic assay that produces a visual readout in the presence of NS1. The assay identified the four dengue serotypes and Zika viral infections without cross-reaction when testing human serum samples from endemic areas in Central and South America and India. This approach could be useful for developing rapid diagnostics for other emerging pathogens.

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A low-cost, equipment-free rapid antigen test distinguishes dengue virus serotypes and Zika virus in patient sera without detectable cross-reactivity.

Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum

Distinguishing dengue from Zika

Science Translational Medicine

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