Research ArticleZIKA VIRUS

Rapid and specific detection of Asian- and African-lineage Zika viruses

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Science Translational Medicine  03 May 2017:
Vol. 9, Issue 388, eaag0538
DOI: 10.1126/scitranslmed.aag0538

LAMP shines light on Zika virus

Rapid and simple assays to detect infectious agents are key to tracking emerging epidemics. Chotiwan et al. describe a loop-mediated amplification (LAMP) assay that detects Zika virus RNA in human biofluids such as serum and semen as well as in mosquitoes, the insect vector that transmits the disease. This approach successfully distinguished the Asian-lineage Zika virus, associated with the current outbreak in the Americas, from the African-lineage Zika virus. This LAMP assay should enable tracking of the Asian-lineage strain as it moves into new geographical locations. A key advantage of this approach is detection without the need for RNA purification or copying RNA into DNA.

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