Technical CommentsImmunology

Response to Comment on “Diversification of the antigen-specific T cell receptor repertoire after varicella zoster vaccination”

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Science Translational Medicine  22 Mar 2017:
Vol. 9, Issue 382, eaai7891
DOI: 10.1126/scitranslmed.aai7891


  • Fig. 1. Analysis of flow cytometry data.

    CFSE-labeled purified naïve T cells and CTV-labeled peripheral blood mononuclear cells were cultured together in the presence of VZV lysate for 8 days. (A) The scatter plot shows gated live CD4 T cells. A small non-proliferating population of 0.5% stained high for both CFSE and CTV (left). More than 80% of these cells were identified as doublets in the forward scatter–area (FSC-A)–forward scatter–weight (FSC-W) plot (right). (B) The scatter plot shows gated live CD4 T cells after gating for single cells in the FSC-A–FSC-W plot. Single-staining CD4 T cells were gated as shown. The resulting scatter plot identifies proliferating naïve CD4 T cells (CTV-negative, CSFE-low) and proliferating total CD4 T cells (CFSE-negative, CTV-low), as was shown in Fig. 4B in (1).

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