Technical CommentsINFERTILITY

Comment on “Absence of sperm RNA elements correlates with idiopathic male infertility”

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Science Translational Medicine  24 Aug 2016:
Vol. 8, Issue 353, pp. 353tc1
DOI: 10.1126/scitranslmed.aaf2396
  • Fig. 1. Heatmap display for all exons of 17 different germ cell and somatic markers.

    The expression of all exons in the 72 samples from (1) is displayed with heatmap colors in the interval [0, 1], as given by the percentile ranks (“Perc. rank”) in data set GSE65683 (GEO). A strong expression of prostate and seminal vesicle markers TGM4/MSMB/SEMG1/SEMG2 is observable in all samples, and Cluster 2 (yellow) also contains the epithelial and leukocyte markers CDH1 and IL8, respectively. Samples were clustered on the basis of all 278,605 features, which resulted in two clearly separable groups (“Cluster”) that do not correlate with the original grouping (“Group”) defined in (1).

Supplementary Materials

  • www.sciencetranslationalmedicine.org/cgi/content/full/8/353/353tc1/DC1

    Theoretical considerations.

    Fig. S1. Round cell concentrations for 100 ejaculates.

    Fig. S2. Analysis of round cell elimination by density gradient centrifugation.

    Fig. S3. Somatic transcript contamination in published gene expression data.

    Fig. S4. Spearman rank correlation matrix for 12 different markers.

    References (1113)

  • Supplementary Material for:

    Comment on "Absence of sperm RNA elements correlates with idiopathic male infertility"

    Heike Cappallo-Obermann and Andrej-Nikolai Spiess*

    *Corresponding author. Email: a.spiess{at}uke.de

    Published 24 August 2016, Sci. Transl. Med. 8, tc1 (2016)
    DOI: 10.1126/scitranslmed.aaf2396

    This PDF file includes:

    • Theoretical considerations.
    • Fig. S1. Round cell concentrations for 100 ejaculates.
    • Fig. S2. Analysis of round cell elimination by density gradient centrifugation.
    • Fig. S3. Somatic transcript contamination in published gene expression data.
    • Fig. S4. Spearman rank correlation matrix for 12 different markers.
    • References (1113)

    [Download PDF]

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