01 June 2016
Vol 8, Issue 341
  • Contents

    • Perspective

      • What does research reproducibility mean?

        The language and conceptual framework of “research reproducibility” are nonstandard and unsettled across the sciences.

    • Research Articles

      • Phase 1 trial of vocimagene amiretrorepvec and 5-fluorocytosine for recurrent high-grade glioma

        Toca 511 and Toca FC show promising results in treating recurrent high-grade glioma, and a specific molecular signature correlates with treatment-related survival.

      • A genomic approach to therapeutic target validation identifies a glucose-lowering GLP1R variant protective for coronary heart disease

        A missense variant in GLP1R associated with lower fasting glucose levels and protective against T2D is associated with lower risk of coronary heart disease, suggesting that GLP1R agonists are not associated with an unacceptable increase in cardiovascular risk.

      • Direct measurement of T cell receptor affinity and sequence from naïve antiviral T cells

        Direct assessment of TCR affinity and correlated TCR sequence from primary human antigen-specific T cells using iTAST reveals a large range of TCR affinity on HCV precursor T cells that varies with age.

    • Editors' Choice

      • The good and bad of T cell promiscuity

        A conserved minimal binding motif permits promiscuous TCR binding for pathogen/self cross-reactivity.

      • Made-by-measure

        Single-cell RNA-Seq can be used to rationally guide the selection of precision cancer therapies, as demonstrated in renal cell carcinoma.

    • Erratum

About The Cover

Cover image expansion

ONLINE COVER Viruses: A Doctor's Littlest Helpers. A new study by Cloughesy et al. demonstrates the potential of harnessing a virus to deliver therapy directly to brain tumors. The virus used in this study infected the cancer cells and delivered the gene for an enzyme called cytosine deaminase, after which the patients were also treated with an inactive drug called 5-FC. Cytosine deaminase converted 5-FC to its active form, 5-FU, which then killed the cancer cells. This image shows virus (green) spreading through the tumor (pink), with viral envelope proteins visible on the surface of infected cancer cells and yellow bits of viral DNA integrated into the cells' DNA. The yellow shapes in the cells' cytoplasm represent cytosine deaminase, the active enzyme. [CREDIT: IDEAMACHINE STUDIO]