Research ArticleInfectious Disease

A gene deletion that up-regulates viral gene expression yields an attenuated RSV vaccine with improved antibody responses in children

See allHide authors and affiliations

Science Translational Medicine  04 Nov 2015:
Vol. 7, Issue 312, pp. 312ra175
DOI: 10.1126/scitranslmed.aac8463

eLetters is an online forum for ongoing peer review. Submission of eLetters are open to all. Please read our Terms of Service before submitting your own eLetter.

Compose eLetter

Plain text

  • Plain text
    No HTML tags allowed.
  • Web page addresses and e-mail addresses turn into links automatically.
  • Lines and paragraphs break automatically.
Author Information
First or given name, e.g. 'Peter'.
Your last, or family, name, e.g. 'MacMoody'.
Your email address, e.g.
Your role and/or occupation, e.g. 'Orthopedic Surgeon'.
Your organization or institution (if applicable), e.g. 'Royal Free Hospital'.
Statement of Competing Interests

This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

Vertical Tabs

  • A stepping stone towards next generation RSV vaccine

    Human respiratory syncytial virus (HRSV) causing severe respiratory infection in infants and children’s belongs to pneumovirus genus in paramyxoviridae family. It is a negative-sense, single-stranded RNA viruses classified into HSRV-A (10 genotypes GA1-7, NA1-2 and SSA1) and HSRV-B (14 genotypes GB1-4, SAB1-3, and BA1-6) groups; have adaptive advantage over host immune system through mutation and nucleotide duplication. The 15kb genome of HSV containing 10 genes encodes for 11 proteins (NS1, NS2, N, M, P, G, F, SH, M2-1, M2-2 and L) sequentially from NS1 to L genes with two ORF for M2 protein (1). Within the host NS1 and NS2 inhibit type-I interferons (IFNs) signalling, M2-1 and M2-2 encodes matrix proteins required for virus assembly contains CD8 epitopes, SH, S and F encodes for viral coat and G is a surface glycoprotein, L encodes for RNA polymerase whereas P is a cofactor for L (1). In order to combat over RSV generated illness a live attenuated RSV vaccine has been considered as an attractive strategy for immunization of infants and children’s beyond their neonatal period. However administration of live attenuated RSV fails to induce sufficient immune response. In such case reverse genetics can laid the foundation of development of engineered virus. Matrix protein M2-2 is responsible for virus assembly, where its depletion restricts virus assembly or virus replication. In such a case enhanced transcription of viral genes elicits substantial humoral and cellular immun...

    Show More
    Competing Interests: None declared.

Stay Connected to Science Translational Medicine

Navigate This Article