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Efficient modification of CCR5 in primary human hematopoietic cells using a megaTAL nuclease and AAV donor template

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Science Translational Medicine  30 Sep 2015:
Vol. 7, Issue 307, pp. 307ra156
DOI: 10.1126/scitranslmed.aac5530

Article Information

vol. 7 no. 307 307ra156

PubMed: 
Print ISSN: 
Online ISSN: 
History: 
  • Received for publication May 11, 2015
  • Accepted for publication September 2, 2015
  • .

Author Information

  1. Blythe D. Sather1,*,
  2. Guillermo S. Romano Ibarra1,*,
  3. Karen Sommer1,
  4. Gabrielle Curinga1,
  5. Malika Hale1,
  6. Iram F. Khan1,
  7. Swati Singh1,
  8. Yumei Song1,
  9. Kamila Gwiazda1,
  10. Jaya Sahni1,
  11. Jordan Jarjour2,
  12. Alexander Astrakhan2,
  13. Thor A. Wagner3,4,
  14. Andrew M. Scharenberg1,4,5, and
  15. David J. Rawlings1,4,5,
  1. 1Center for Immunity and Immunotherapies and Program for Cell and Gene Therapy, Seattle Children’s Research Institute, Seattle, WA 98101, USA.
  2. 2Bluebird Bio, Seattle, WA 98102, USA.
  3. 3Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA 98101, USA.
  4. 4Department of Pediatrics, University of Washington, Seattle, WA 98101, USA.
  5. 5Department of Immunology, University of Washington, Seattle, WA 98101, USA.
  1. Corresponding author. E-mail: andrewms{at}u.washington.edu (A.M.S.); drawling{at}uw.edu (D.J.R.)
    • * These authors contributed equally.

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