Research ArticleImmunology

High-throughput pairing of T cell receptor α and β sequences

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Science Translational Medicine  19 Aug 2015:
Vol. 7, Issue 301, pp. 301ra131
DOI: 10.1126/scitranslmed.aac5624

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T cell receptor chains pair off

High-throughput immunosequencing can take a snapshot of the repertoire of immune cells, providing a broad picture of the immune response at any given time and tracking how the immune response changes as a result of perturbations such as vaccines, infection, or cancer. However, this approach has been limited by the inability to determine which TCR α and TCR β chains combine to form specific T cell receptors in a given cell. Now, Howie et al. report and validate a high-throughput method to pair TCR α and β segments without the need for single-cell technologies. They confirm that their method can be used for T cells from both blood and solid tissues.

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