TCR-Ligand koff Rate Correlates with the Protective Capacity of Antigen-Specific CD8+ T Cells for Adoptive Transfer

Magdalena Nauerth; Bianca Weißbrich; Robert Knall; Tobias Franz; Georg Dössinger; Jeannette Bet; Paulina J. Paszkiewicz; Lukas Pfeifer; Mario Bunse; Wolfgang Uckert; Rafaela Holtappels; Dorothea Gillert-Marien; Michael Neuenhahn; Angela Krackhardt; Matthias J. Reddehase; Stanley R. Riddell; Dirk H. Busch

doi:10.1126/scitranslmed.3005958

Research ArticleImmunology

TCR-Ligand k_off Rate Correlates with the Protective Capacity of Antigen-Specific CD8⁺ T Cells for Adoptive Transfer

Magdalena Nauerth¹,*,
Bianca Weißbrich¹,*,
Robert Knall¹,*,
Tobias Franz¹,
Georg Dössinger¹,
Jeannette Bet¹,²,
Paulina J. Paszkiewicz¹,²,
Lukas Pfeifer¹,
Mario Bunse³,⁴,
Wolfgang Uckert³,⁴,
Rafaela Holtappels⁵,
Dorothea Gillert-Marien⁵,
Michael Neuenhahn¹,⁶,⁷,
Angela Krackhardt⁶,⁸,
Matthias J. Reddehase⁵,⁹,
Stanley R. Riddell²,¹⁰,¹¹ and
Dirk H. Busch¹,²,⁶,⁷,†

¹Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.
²Focus Group “Clinical Cell Processing and Purification,” Institute for Advanced Study, Technische Universität München, 81675 Munich, Germany.
³Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
⁴Humboldt-Universität Berlin, 10099 Berlin, Germany.
⁵Institute for Virology and Research Center for Immunology (FZI), University Medical Center of the Johannes Gutenberg-University, 55131 Mainz, Germany.
⁶Clinical Cooperation Groups “Antigen-specific Immunotherapy” and “Immune Monitoring,” Helmholtz Center Munich (85764 Neuherberg) and Technische Universität München, 81675 Munich, Germany.
⁷DZIF–National Centre for Infection Research, 81675 Munich, Germany.
⁸Medical Department III, Hematology and Oncology, Technische Universität München, 81675 Munich, Germany.
⁹Helmholtz Virtual Institute on Viral Strategies of Immune Evasion (VISTRIE), Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.
¹⁰Program in Immunology, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
¹¹Department of Medicine, University of Washington, Seattle, WA 98104, USA.

↵†Corresponding author. E-mail: dirk.busch{at}mikrobio.med.tum.de

↵* These authors contributed equally to this work.

See allHide authors and affiliations

Science Translational Medicine 03 Jul 2013:
Vol. 5, Issue 192, pp. 192ra87
DOI: 10.1126/scitranslmed.3005958

Magdalena Nauerth

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Bianca Weißbrich

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Robert Knall

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Tobias Franz

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Georg Dössinger

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Jeannette Bet

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

2Focus Group “Clinical Cell Processing and Purification,” Institute for Advanced Study, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Paulina J. Paszkiewicz

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

2Focus Group “Clinical Cell Processing and Purification,” Institute for Advanced Study, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Lukas Pfeifer

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Mario Bunse

3Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.

4Humboldt-Universität Berlin, 10099 Berlin, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Wolfgang Uckert

3Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.

4Humboldt-Universität Berlin, 10099 Berlin, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Rafaela Holtappels

5Institute for Virology and Research Center for Immunology (FZI), University Medical Center of the Johannes Gutenberg-University, 55131 Mainz, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Dorothea Gillert-Marien

5Institute for Virology and Research Center for Immunology (FZI), University Medical Center of the Johannes Gutenberg-University, 55131 Mainz, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Michael Neuenhahn

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

6Clinical Cooperation Groups “Antigen-specific Immunotherapy” and “Immune Monitoring,” Helmholtz Center Munich (85764 Neuherberg) and Technische Universität München, 81675 Munich, Germany.

7DZIF–National Centre for Infection Research, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Angela Krackhardt

6Clinical Cooperation Groups “Antigen-specific Immunotherapy” and “Immune Monitoring,” Helmholtz Center Munich (85764 Neuherberg) and Technische Universität München, 81675 Munich, Germany.

8Medical Department III, Hematology and Oncology, Technische Universität München, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Matthias J. Reddehase

5Institute for Virology and Research Center for Immunology (FZI), University Medical Center of the Johannes Gutenberg-University, 55131 Mainz, Germany.

9Helmholtz Virtual Institute on Viral Strategies of Immune Evasion (VISTRIE), Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Stanley R. Riddell

2Focus Group “Clinical Cell Processing and Purification,” Institute for Advanced Study, Technische Universität München, 81675 Munich, Germany.

10Program in Immunology, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Dirk H. Busch

1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81675 Munich, Germany.

2Focus Group “Clinical Cell Processing and Purification,” Institute for Advanced Study, Technische Universität München, 81675 Munich, Germany.

6Clinical Cooperation Groups “Antigen-specific Immunotherapy” and “Immune Monitoring,” Helmholtz Center Munich (85764 Neuherberg) and Technische Universität München, 81675 Munich, Germany.

7DZIF–National Centre for Infection Research, 81675 Munich, Germany.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
For correspondence: dirk.busch@mikrobio.med.tum.de

Article
Figures & Data
Info & Metrics
eLetters
PDF

eLetters is an online forum for ongoing peer review. Submission of eLetters are open to all. Please read our Terms of Service before submitting your own eLetter.

Submit a Response to This Article

Compose eLetter

Title

Contents

More information about text formats

Plain text

Plain text
No HTML tags allowed.
Web page addresses and e-mail addresses turn into links automatically.
Lines and paragraphs break automatically.

Author Information

Contributors
First name and middle name First or given name, e.g. 'Peter'. Last Name Your last, or family, name, e.g. 'MacMoody'. Email Your email address, e.g. higgs-boson@gmail.com Role / Occupation Your role and/or occupation, e.g. 'Orthopedic Surgeon'. Affiliation Your organization or institution (if applicable), e.g. 'Royal Free Hospital'.

Statement of Competing Interests

Competing interests?

Yes

Please describe the competing interests

CAPTCHA

This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

Vertical Tabs

No eLetters have been published for this article.

View Full Text

TCR-Ligand k_off Rate Correlates with the Protective Capacity of Antigen-Specific CD8⁺ T Cells for Adoptive Transfer

Plain text

Vertical Tabs

Science Translational Medicine

Article Tools