Response to Comment on “Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells”

Naohiro Egawa; Shiho Kitaoka; Kayoko Tsukita; Motoko Naitoh; Kazutoshi Takahashi; Takuya Yamamoto; Fumihiko Adachi; Takayuki Kondo; Keisuke Okita; Isao Asaka; Takashi Aoi; Akira Watanabe; Yasuhiro Yamada; Asuka Morizane; Jun Takahashi; Takashi Ayaki; Hidefumi Ito; Katsuhiro Yoshikawa; Satoko Yamawaki; Shigehiko Suzuki; Dai Watanabe; Hiroyuki Hioki; Takeshi Kaneko; Kouki Makioka; Koichi Okamoto; Hiroshi Takuma; Akira Tamaoka; Kazuko Hasegawa; Takashi Nonaka; Masato Hasegawa; Akihiro Kawata; Minoru Yoshida; Tatsutoshi Nakahata; Ryosuke Takahashi; Maria C. N. Marchetto; Fred H. Gage; Shinya Yamanaka; Haruhisa Inoue

doi:10.1126/scitranslmed.3005697

LetterStem Cells

Response to Comment on “Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells”

Naohiro Egawa¹,²,*,
Shiho Kitaoka¹,²,*,
Kayoko Tsukita¹,²,
Motoko Naitoh³,
Kazutoshi Takahashi¹,
Takuya Yamamoto¹,⁴,
Fumihiko Adachi¹,
Takayuki Kondo¹,⁵,
Keisuke Okita¹,
Isao Asaka¹,
Takashi Aoi¹,
Akira Watanabe¹,⁴,
Yasuhiro Yamada¹,⁴,
Asuka Morizane¹,⁶,
Jun Takahashi¹,⁶,
Takashi Ayaki⁵,
Hidefumi Ito⁵,
Katsuhiro Yoshikawa³,
Satoko Yamawaki³,
Shigehiko Suzuki³,
Dai Watanabe⁷,⁸,
Hiroyuki Hioki⁹,
Takeshi Kaneko⁹,
Kouki Makioka¹⁰,
Koichi Okamoto¹⁰,
Hiroshi Takuma¹¹,
Akira Tamaoka¹¹,
Kazuko Hasegawa¹²,
Takashi Nonaka¹³,
Masato Hasegawa¹³,
Akihiro Kawata¹⁴,
Minoru Yoshida¹⁵,
Tatsutoshi Nakahata¹,
Ryosuke Takahashi⁵,
Maria C. N. Marchetto¹⁶,
Fred H. Gage¹⁶,
Shinya Yamanaka¹,⁴,¹⁷ and
Haruhisa Inoue¹,²,¹⁷,^†

¹Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
²CREST, Japan Science and Technology Agency (JST), Tokyo 332-0012, Japan.
³Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
⁴Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto 606-8501, Japan.
⁵Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
⁶Department of Biological Repair, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
⁷Department of Biological Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
⁸Department of Molecular and Systems Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
⁹Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
¹⁰Department of Neurology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.
¹¹Department of Neurology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba 305-8576, Japan.
¹²Department of Neurology, Sagamihara Hospital, Sagamihara 252-0392, Japan.
¹³Department of Neuropathology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.
¹⁴Department of Neurology, Tokyo Metropolitan Neurological Hospital, Tokyo 183-0042, Japan.
¹⁵Chemical Genetics Laboratory Molecular Ligand Biology Research Team, Chemical Genomics Research Group, RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
¹⁶The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
¹⁷Yamanaka iPS Cell Special Project, JST, Kawaguchi 332-0012, Japan.

↵†Corresponding author. E-mail: haruhisa{at}cira.kyoto-u.ac.jp

↵* These authors contributed equally to this work.

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Science Translational Medicine 05 Jun 2013:
Vol. 5, Issue 188, pp. 188lr2
DOI: 10.1126/scitranslmed.3005697

Naohiro Egawa

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

2CREST, Japan Science and Technology Agency (JST), Tokyo 332-0012, Japan.

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Shiho Kitaoka

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

2CREST, Japan Science and Technology Agency (JST), Tokyo 332-0012, Japan.

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Kayoko Tsukita

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

2CREST, Japan Science and Technology Agency (JST), Tokyo 332-0012, Japan.

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Motoko Naitoh

3Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Kazutoshi Takahashi

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

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Takuya Yamamoto

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

5Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Fumihiko Adachi

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

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Takayuki Kondo

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

4Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto 606-8501, Japan.

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Keisuke Okita

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

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Isao Asaka

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

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Takashi Aoi

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

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Akira Watanabe

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

5Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Yasuhiro Yamada

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

5Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Asuka Morizane

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

6Department of Biological Repair, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.

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Jun Takahashi

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

6Department of Biological Repair, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.

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Takashi Ayaki

4Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto 606-8501, Japan.

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Hidefumi Ito

4Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto 606-8501, Japan.

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Katsuhiro Yoshikawa

3Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Satoko Yamawaki

3Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Shigehiko Suzuki

3Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Dai Watanabe

7Department of Biological Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

8Department of Molecular and Systems Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.

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Hiroyuki Hioki

9Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

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Takeshi Kaneko

9Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

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Kouki Makioka

10Department of Neurology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.

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Koichi Okamoto

10Department of Neurology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.

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Hiroshi Takuma

11Department of Neurology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba 305-8576, Japan.

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Akira Tamaoka

11Department of Neurology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba 305-8576, Japan.

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Kazuko Hasegawa

12Department of Neurology, Sagamihara Hospital, Sagamihara 252-0392, Japan.

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Takashi Nonaka

13Department of Neuropathology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.

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Masato Hasegawa

13Department of Neuropathology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.

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Akihiro Kawata

14Department of Neurology, Tokyo Metropolitan Neurological Hospital, Tokyo 183-0042, Japan.

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Minoru Yoshida

15Chemical Genetics Laboratory Molecular Ligand Biology Research Team, Chemical Genomics Research Group, RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.

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Tatsutoshi Nakahata

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

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Ryosuke Takahashi

5Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

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Maria C. N. Marchetto

16The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

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Fred H. Gage

16The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

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Shinya Yamanaka

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

5Department of Neurology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

17Yamanaka iPS Cell Special Project, JST, Kawaguchi 332-0012, Japan.

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Haruhisa Inoue

1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

2CREST, Japan Science and Technology Agency (JST), Tokyo 332-0012, Japan.

17Yamanaka iPS Cell Special Project, JST, Kawaguchi 332-0012, Japan.

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For correspondence: haruhisa@cira.kyoto-u.ac.jp

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Abstract

Our work and the study of Bilican et al. highlight the need for complementary assays to detect subtle phenotypic differences between control and mutant induced pluripotent stem cell lines.

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Response to Comment on “Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells”

Abstract

Science Translational Medicine

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