Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining

E. Lake Potter; Hannah P. Gideon; Victor Tkachev; Giulia Fabozzi; Alexander Chassiakos; Constantinos Petrovas; Patricia A. Darrah; Philana Ling Lin; Kathryn E. Foulds; Leslie S. Kean; JoAnne L. Flynn; Mario Roederer

doi:10.1126/scitranslmed.abb4582

Research ResourceImmunology

Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining

View ORCID ProfileE. Lake Potter¹,
View ORCID ProfileHannah P. Gideon ²,
View ORCID ProfileVictor Tkachev ³,⁴,
Giulia Fabozzi¹,
View ORCID ProfileAlexander Chassiakos¹,
View ORCID ProfileConstantinos Petrovas¹,
Patricia A. Darrah¹,
Philana Ling Lin ⁵,
View ORCID ProfileKathryn E. Foulds¹,
View ORCID ProfileLeslie S. Kean ³,⁴,
View ORCID ProfileJoAnne L. Flynn ² and
View ORCID ProfileMario Roederer ¹,*

¹Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
²Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA.
³Boston Children’s Hospital, Division of Hematology/Oncology, Boston, MA 02115, USA.
⁴Dana-Farber Cancer Institute, Department of Pediatric Oncology and Harvard Medical School, Boston, MA 02215, USA.
⁵Department of Pediatrics, University of Pittsburgh Medical Center Children’s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA.

↵*Corresponding author. Email: roederer{at}nih.gov

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Science Translational Medicine 13 Jan 2021:
Vol. 13, Issue 576, eabb4582
DOI: 10.1126/scitranslmed.abb4582

E. Lake Potter

1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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Hannah P. Gideon

2Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA.

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Victor Tkachev

3Boston Children’s Hospital, Division of Hematology/Oncology, Boston, MA 02115, USA.

4Dana-Farber Cancer Institute, Department of Pediatric Oncology and Harvard Medical School, Boston, MA 02215, USA.

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Giulia Fabozzi

1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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Alexander Chassiakos

1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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ORCID record for Alexander Chassiakos

Constantinos Petrovas

1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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Patricia A. Darrah

1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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Philana Ling Lin

5Department of Pediatrics, University of Pittsburgh Medical Center Children’s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA.

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Kathryn E. Foulds

1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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Leslie S. Kean

3Boston Children’s Hospital, Division of Hematology/Oncology, Boston, MA 02115, USA.

4Dana-Farber Cancer Institute, Department of Pediatric Oncology and Harvard Medical School, Boston, MA 02215, USA.

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JoAnne L. Flynn

2Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA.

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Mario Roederer

1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

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For correspondence: roederer@nih.gov

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Serial intravascular staining enables analysis of leukocyte migration in healthy nonhuman primates and those infected with Mycobacterium tuberculosis.

Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining

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