Research ResourceImmunology

Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining

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Science Translational Medicine  13 Jan 2021:
Vol. 13, Issue 576, eabb4582
DOI: 10.1126/scitranslmed.abb4582

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SIVSing through GVHD

Currently, there are few ways to track leukocyte movements in vivo. Potter and colleagues developed a method to track leukocyte migration in vivo called serial intravascular staining or SIVS. The authors infused differently labeled antibodies at different time points to reveal distinct leukocyte population kinetics in healthy macaques and those infected with Mycobacterium tuberculosis. Tkachev et al. then applied SIVS to shed light on donor T cell trafficking in macaques that developed acute graft-versus-host disease (GVHD) after transplant. They found that donor cytotoxic CD8+ T cells infiltrated the gastrointestinal tract and developed the transcriptional signature of tissue-resident memory T cells. SIVS will be useful for revealing leukocyte trafficking kinetics not only in GVHD and infection but in a variety of other diseases as well.

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