13 January 2021
Vol 13, Issue 576

About The Cover

Cover image expansion

ONLINE COVER Leukocytes on the Run. This image shows the migration of blood leukocytes into a rhesus macaque lymph node. Using a technique termed serial intravascular staining (SIVS), Potter et al. intravenously administered anti-CD45 antibody to label blood leukocytes at 6 hours, 2 hours, and 5 minutes before necropsy, represented by red, teal, and green fluorescence, respectively. This serial staining produced a picture of migration into the lymph node over time. In a paired study by Tkachev et al., SIVS was used in combination with single cell RNA sequencing to characterize the role of donor CD8+ T cells in graft-versus-host disease in macaques. Thus, SIVS promises to improve our understanding of the dynamics of leukocyte trafficking in vivo. [CREDIT: E. LAKE POTTER AND GUILIA FABOZZI]