A genome-wide CRISPR-based screen identifies KAT7 as a driver of cellular senescence

Wei Wang; Yuxuan Zheng; Shuhui Sun; Wei Li; Moshi Song; Qianzhao Ji; Zeming Wu; Zunpeng Liu; Yanling Fan; Feifei Liu; Jingyi Li; Concepcion Rodriguez Esteban; Si Wang; Qi Zhou; Juan Carlos Izpisua Belmonte; Weiqi Zhang; Jing Qu; Fuchou Tang; Guang-Hui Liu

doi:10.1126/scitranslmed.abd2655

Research ArticleAging

A genome-wide CRISPR-based screen identifies KAT7 as a driver of cellular senescence

View ORCID ProfileWei Wang¹,²,³,⁴,*,
View ORCID ProfileYuxuan Zheng⁵,⁶,*,
View ORCID ProfileShuhui Sun¹,³,⁴,*,
View ORCID ProfileWei Li⁷,*,
Moshi Song¹,⁴,⁸,
Qianzhao Ji¹,⁴,
Zeming Wu²,⁴,
Zunpeng Liu²,⁴,
Yanling Fan⁹,¹⁰,
Feifei Liu¹,
View ORCID ProfileJingyi Li¹,⁴,
Concepcion Rodriguez Esteban¹¹,
View ORCID ProfileSi Wang¹,⁷,⁸,
View ORCID ProfileQi Zhou²,⁴,⁸,
View ORCID ProfileJuan Carlos Izpisua Belmonte¹¹,
View ORCID ProfileWeiqi Zhang⁴,⁷,⁸,⁹,¹⁰,†,
View ORCID ProfileJing Qu²,⁴,⁸,†,
View ORCID ProfileFuchou Tang⁵,⁶,† and
View ORCID ProfileGuang-Hui Liu¹,³,⁴,⁷,⁸,†

¹State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
²State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
³National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
⁴University of Chinese Academy of Sciences, Beijing 100049, China.
⁵Beijing Advanced Innovation Center for Genomics, Biomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China.
⁶Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
⁷Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China.
⁸Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.
⁹CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
¹⁰China National Center for Bioinformation, Chinese Academy of Sciences, Beijing 100101, China.
¹¹Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

↵†Corresponding author. Email: ghliu{at}ioz.ac.cn (G.-H.L.); tangfuchou{at}pku.edu.cn (F.T.); qujing{at}ioz.ac.cn (J.Q.); zhangwq{at}big.ac.cn (W.Z.)

↵* These authors contributed equally to this work.

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Science Translational Medicine 06 Jan 2021:
Vol. 13, Issue 575, eabd2655
DOI: 10.1126/scitranslmed.abd2655

Wei Wang

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

2State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

3National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

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Yuxuan Zheng

5Beijing Advanced Innovation Center for Genomics, Biomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China.

6Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

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ORCID record for Yuxuan Zheng

Shuhui Sun

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

3National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

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Wei Li

7Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China.

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ORCID record for Wei Li

Moshi Song

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

8Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

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Qianzhao Ji

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

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Zeming Wu

2State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

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Zunpeng Liu

2State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

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Yanling Fan

9CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.

10China National Center for Bioinformation, Chinese Academy of Sciences, Beijing 100101, China.

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Feifei Liu

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

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Jingyi Li

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

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Concepcion Rodriguez Esteban

11Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

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Si Wang

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

7Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China.

8Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

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Qi Zhou

2State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

8Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

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Juan Carlos Izpisua Belmonte

11Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

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Weiqi Zhang

4University of Chinese Academy of Sciences, Beijing 100049, China.

7Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China.

8Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

9CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.

10China National Center for Bioinformation, Chinese Academy of Sciences, Beijing 100101, China.

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For correspondence: ghliu@ioz.ac.cn tangfuchou@pku.edu.cn qujing@ioz.ac.cn zhangwq@big.ac.cn

Jing Qu

2State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

8Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

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ORCID record for Jing Qu
For correspondence: ghliu@ioz.ac.cn tangfuchou@pku.edu.cn qujing@ioz.ac.cn zhangwq@big.ac.cn

Fuchou Tang

5Beijing Advanced Innovation Center for Genomics, Biomedical Pioneering Innovation Center, School of Life Sciences, Peking University, Beijing 100871, China.

6Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

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For correspondence: ghliu@ioz.ac.cn tangfuchou@pku.edu.cn qujing@ioz.ac.cn zhangwq@big.ac.cn

Guang-Hui Liu

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

3National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

4University of Chinese Academy of Sciences, Beijing 100049, China.

7Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital Capital Medical University, Beijing 100053, China.

8Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

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ORCID record for Guang-Hui Liu
For correspondence: ghliu@ioz.ac.cn tangfuchou@pku.edu.cn qujing@ioz.ac.cn zhangwq@big.ac.cn

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A genome-wide CRISPR-based screen identifies KAT7 as a driver of cellular senescence

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